Effects of pentoxifylline on TNF-alpha and lung histopathology in HCL-induced lung injury

Effects of pentoxifylline on TNF-alpha and lung histopathology in HCL-induced lung injury

Author Oliveira-Júnior, Itamar Souza Autor UNIFESP Google Scholar
Maganhin, Carla Cristina Autor UNIFESP Google Scholar
Carbonel, Adriana Aparecida Ferraz Autor UNIFESP Google Scholar
Monteiro, Cristina Maria Rodrigues Autor UNIFESP Google Scholar
Cavassani, Sâmia Santos Autor UNIFESP Google Scholar
Oliveira-filho, Ricardo Martins Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE: To evaluate the effects of pentoxifylline on hydrochloric acid-induced lung lesions in rats subjected to mechanical ventilation. METHODS: Twenty male, adult Wistar-EPM-1 rats were anesthetized and randomly grouped (n=5 animals per group) as follows: control-MV (mechanical ventilation, MV group); bilateral instillation of HCl (HCl group); bilateral instillation of HCl followed by pentoxifylline (50 mg/kg bw) infusion (HCl+PTX group) and pentoxifylline infusion followed by bilateral instillation of HCl (PTX+HCl group). At 20, 30, 90 and 180 min after treatments, the blood partial pressures of CO2 and O2 were measured. The animals were euthanized, and bronchoalveolar lavages were taken to determine the contents of total proteins, corticosterone and TNF-alpha. Samples of lung tissue were used for histomorphometric studies and determining the wet-to-dry (W/D) lung weight ratio. RESULTS: In the MV group, rats had alveolar septal congestion, and, in the HCl group, a remarkable recruitment of neutrophils and macrophages into the alveoli was noticed; these events were reduced in the animals with PTX+HCl. The partial pressure of oxygen increased in PTX+HCl animals (121±5 mmHg) as compared with the HCl (62±6 mmHg) and HCl+PTX (67±3 mmHg) groups within 30 minutes. TNF-alpha levels in bronchoalveolar lavage were significantly higher in the HCl group (458±50 pg/mL), reduced in the HCl+PTX group (329±45 pg/mL) and lowest in the PTX+HCl group (229±41 pg/mL). The levels of corticosterone in bronchoalveolar lavage were significantly lower in the HCl (8±1.3 ng/mL) and HCl+PTX group (16±2 ng/mL) and were highest in the PTX+HCl (27±1.9 ng/mL). CONCLUSION: Pretreatment with PTX improves oxygenation, reduces TNF-alpha concentration and increases the concentration of corticosterone in bronchoalveolar lavage upon lung lesion induced by HCl.
Keywords Acid aspiration
Acute lung injury
Mechanical ventilation
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2008-01-01
Published in Clinics. Faculdade de Medicina / USP, v. 63, n. 1, p. 77-84, 2008.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Faculdade de Medicina / USP
Extent 77-84
Origin http://dx.doi.org/10.1590/S1807-59322008000100014
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000254321700014
SciELO ID S1807-59322008000100014 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/4158

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