Effects of the angiotensin II Ala-scan analogs in erythrocytic cycle of Plasmodium falciparum (in vitro) and Plasmodium gallinaceum (ex vivo)

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Silva, Adriana Farias
Silva, Leandro de Souza
Alves, Flavio Lopes [UNIFESP]
TorossianTorres, Marcelo Der
SaPinheiro, Ana Acacia de
Miranda, Antonio
LaraCapurro, Margareth
Oliveira, Vani Xavier
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The anti-plasmodium activity of angiotensin II and its analogs have been described in different plasmodium species. Here we synthesized angiotensin II Ala-scan analogs to verify peptide-parasite invasion preservation with residue replacements. the analogs were synthesized by 9-fluorenylmethoxycarbonyl (Fmoc) and tert-butyloxycarbonyl (t-Boc) solid phase methods, purified by liquid chromatography and characterized by mass spectrometry. the results obtained in Plasmodium falciparum assays indicated that all analogs presented some influence in parasite invasion, except [Ala(4)]-Ang II (18% of anti-plasmodium activity) that was not statistically different from control. Although [Ala(8)]-Ang II presented a lower biological activity (20%), it was statistically different from control. the most relevant finding was that [Ala(5)]-Ang II preserved activity (45%) relative to Ang II (47%). in the results of Plasmodium gallinaceum assays all analogs were not statistically different from control, except [Ala(6)]-Ang II, which was able to reduce the parasitemia about 49%. This approach provides insight for understanding the importance of each amino acid on the native Ang II sequence and provides a new direction for the design of potential chemotherapeutic agents without pressor activity. (C) 2015 Elsevier Inc. All rights reserved.
Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 153, p. 1-7, 2015.