Binding of Methylene Blue onto Langmuir Mono layers Representing Cell Membranes May Explain Its Efficiency as Photosensitizer in Photodynamic Therapy
Nenhuma Miniatura disponível
Data
2015-04-14
Autores
Schmidt, Thais F.
Caseli, Luciano [UNIFESP]
Oliveira, Osvaldo N.
Itri, Rosangela
Orientadores
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
We provide evidence for the electrostatic interactions between the cationic photosensitizer methylene blue (MB) and cell membrane models represented by neat and mixed Langmuir monolayers of dioleylphosphatidylcholine (DOPC) and 1,1',2,2'-tetraoleoylcardiolipin (CL). From surface pressure measurements, MB was found to adsorb strongly and expand CL-containing monolayers, while it caused an apparent decreasing in molecular area on neat DOPC monolayer. the binding site of MB could be inferred from data with the surface-specific polarization-modulated infrared reflectionabsorption spectroscopy (PM-IRRAS) technique, where changes induced by MB were observed in the vibrational modes of the phosphate groups of both CL and DOPC. the incorporation of MB also affected the carbonyl groups and the packing of the alkyl chains, thus indicating that MB binding site favors singlet oxygen generation close to the double bonds in the alkyl chains, an important requirement for photodynamic efficiency. Significantly, the data presented here demonstrate that MB may act in membranes composed by PCs, such as mammalian plasma membranes, and in those containing CL, as in bacterial and inner mitochondrial membranes.
Descrição
Citação
Langmuir. Washington: Amer Chemical Soc, v. 31, n. 14, p. 4205-4212, 2015.