Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags

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Date
2007-09-01
Author
Gentil, Luciana Girotto [UNIFESP]
Lasakosvitsch, Fernanda [UNIFESP]
Franco da Silveira, José [UNIFESP]
Santos, Márcia Regina Machado dos [UNIFESP]
Barbiéri, Clara Lúcia [UNIFESP]
Type
Artigo
ISSN
0074-0276
Is part of
Memórias do Instituto Oswaldo Cruz
DOI
10.1590/S0074-02762007005000065
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Abstract
The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.
Citation
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 6, p. 707-711, 2007.
Keywords
Leishmania (Leishmania) amazonensis
amastigotes
cDNA library
URI
http://repositorio.unifesp.br/handle/11600/3894
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