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dc.contributor.authorLun, Melody P.
dc.contributor.authorJohnson, Matthew B.
dc.contributor.authorBroadbelt, Kevin G.
dc.contributor.authorWatanabe, Momoko
dc.contributor.authorKang, Young-jin
dc.contributor.authorChau, Kevin F.
dc.contributor.authorSpringel, Mark W.
dc.contributor.authorMalesz, Alexandra
dc.contributor.authorSousa, Andre M. M.
dc.contributor.authorPletikos, Mihovil
dc.contributor.authorAdelita, Tai [UNIFESP]
dc.contributor.authorCalicchio, Monica L.
dc.contributor.authorZhang, Yong
dc.contributor.authorHoltzman, Michael J.
dc.contributor.authorLidov, Hart G. W.
dc.contributor.authorSestan, Nenad
dc.contributor.authorSteen, Hanno
dc.contributor.authorMonuki, Edwin S.
dc.contributor.authorLehtinen, Maria K.
dc.identifier.citationJournal of Neuroscience. Washington: Soc Neuroscience, v. 35, n. 12, p. 4903-4916, 2015.
dc.description.abstractA sheet of choroid plexus epithelial cells extends into each cerebral ventricle and secretes signaling factors into the CSF. To evaluate whether differences in the CSF proteome across ventricles arise, in part, from regional differences in choroid plexus gene expression, we defined the transcriptome of lateral ventricle (telencephalic) versus fourth ventricle (hindbrain) choroid plexus. We find that positional identities of mouse, macaque, and human choroid plexi derive from gene expression domains that parallel their axial tissues of origin. We then show that molecular heterogeneity between telencephalic and hindbrain choroid plexi contributes to region-specific, age-dependent protein secretion in vitro. Transcriptome analysis of FACS-purified choroid plexus epithelial cells also predicts their cell-type-specific secretome. Spatial domains with distinct protein expression profiles were observed within each choroid plexus. We propose that regional differences between choroid plexi contribute to dynamic signaling gradients across the mammalian cerebroventricular system.en
dc.description.sponsorshipABTA Medical Student Summer Fellowship
dc.description.sponsorshipGlenn/AFAR Scholarship for Research in the Biology of Aging
dc.description.sponsorshipNancy Lurie Marks Family Foundation Postdoctoral Fellowship
dc.description.sponsorshipCalifornia Institute for Regenerative Medicine (CIRM) Training Grant
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipUCI Institute for Clinical and Translational Science Pilot Project Award
dc.description.sponsorshipPediatric Hydrocephalus Foundation
dc.description.sponsorshipBCH IDDRC
dc.publisherSoc Neuroscience
dc.relation.ispartofJournal of Neuroscience
dc.rightsAcesso aberto
dc.subjectcerebrospinal fluiden
dc.subjectchoroid plexusen
dc.subjectnext-generation sequencingen
dc.titleSpatially Heterogeneous Choroid Plexus Transcriptomes Encode Positional Identity and Contribute to Regional CSF Productionen
dc.contributor.institutionBoston Childrens Hosp
dc.contributor.institutionBoston Univ
dc.contributor.institutionUniv Calif Irvine
dc.contributor.institutionYale Univ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionWashington Univ
dc.description.affiliationBoston Childrens Hosp, Dept Pathol, Boston, MA 02115 USA
dc.description.affiliationBoston Childrens Hosp, Div Genet, Boston, MA 02115 USA
dc.description.affiliationBoston Univ, Sch Med, Dept Pathol & Lab Med, Boston, MA 02118 USA
dc.description.affiliationUniv Calif Irvine, Sch Med, Dept Pathol & Lab Med, Irvine, CA 92697 USA
dc.description.affiliationYale Univ, Sch Med, Dept Neurobiol, New Haven, CT 06510 USA
dc.description.affiliationYale Univ, Sch Med, Kavli Inst Neurosci, New Haven, CT 06510 USA
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, BR-04039 São Paulo, Brazil
dc.description.affiliationWashington Univ, Dept Med, Pulm & Crit Care Med, St Louis, MO 63110 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, BR-04039 São Paulo, Brazil
dc.description.sponsorshipIDCalifornia Institute for Regenerative Medicine (CIRM) Training Grant: TF2-01152
dc.description.sponsorshipIDFAPESP: 2013/24501-9
dc.description.sponsorshipIDNIH: U19 AI070489
dc.description.sponsorshipIDNIH: MH106934
dc.description.sponsorshipIDNIH: CIRM RN2-00915-1
dc.description.sponsorshipIDPediatric Hydrocephalus Foundation: NIH K99/R00
dc.description.sponsorshipIDPediatric Hydrocephalus Foundation: NS072192
dc.description.sponsorshipIDPediatric Hydrocephalus Foundation: R01 NS088566
dc.description.sponsorshipIDBCH IDDRC: P30 HD18655
dc.description.sourceWeb of Science

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