Culturing Drosophila melanogaster (S2) in a chemostat

Culturing Drosophila melanogaster (S2) in a chemostat

Author Vieira, Paula Bruzadelle Google Scholar
Vale da Costa, Bruno Labate Google Scholar
Pires Augusto, Elisabeth de Fatima Autor UNIFESP Google Scholar
Tonso, Aldo Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Insect cells are used for the expression of complex proteins in products such as vaccines and biopharmaceuticals. Physiology of a Drosophila melanogaster (lineage S2), transfected to stably express rabies virus glycoprotein (RVGP), was studied in batch culture and in a chemostat with serum-free medium. in batch mode, the system reached 3 x 10(7) cells mL(-1) with specific growth rate of 1.5 d(-1) with RVGP at 2.50 A mu g L-1. When operated continuously, three well-defined steady states were achieved at dilution rates (D) of 0.8, 0.5 and 0.2 d(-1). the residual glucose and glutamine concentrations and the cell yields on glucose and glutamine decreased at lower D. High values of substrate consumption for maintenance may explain this variation in yields. the results indicated that glucose is not the limiting substrate of this process.
Keywords Bioprocess engineering
Bioreactor
Continuous culture
Drosophila melanogaster
Insect cell
Rabies virus glycoprotein
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2015-03-01
Published in Biotechnology Letters. Dordrecht: Springer, v. 37, n. 3, p. 533-538, 2015.
ISSN 0141-5492 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 533-538
Origin http://dx.doi.org/10.1007/s10529-014-1717-9
Access rights Closed access
Type Article
Web of Science ID WOS:000350394800006
URI http://repositorio.unifesp.br/handle/11600/38816

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