Aerobic exercise modulation of mental stress-induced responses in cultured endothelial progenitor cells from healthy and metabolic syndrome subjects

Aerobic exercise modulation of mental stress-induced responses in cultured endothelial progenitor cells from healthy and metabolic syndrome subjects

Author Rocha, Natalia G. Google Scholar
Sales, Allan R. K. Google Scholar
Miranda, Renan L. Google Scholar
Silva, Mayra S. Google Scholar
Silva, Jemima F. R. Google Scholar
Silva, Bruno M. Autor UNIFESP Google Scholar
Santos, Aline A. Google Scholar
Nobrega, Antonio C. L. Google Scholar
Institution Universidade Federal Fluminense (UFF)
Universidade Federal de São Paulo (UNIFESP)
Abstract Aim: Numerous studies have demonstrated that exercise acutely prevents the reduction in flow-mediated dilation induced by mental stress in subjects with metabolic syndrome (MetS). However, it is unknown whether a similar effect occurs in endothelial progenitors cells (EPCs). This study investigated whether exercise protects from the deleterious effect of mental stress on cultured EPCs in healthy subjects and those with MetS.Main methods: Ten healthy subjects (aged 31 +/- 2) and ten subjects with MetS (aged 36 +/- 2) were enrolled. Subjects underwent a mental stress test, followed immediately by either 40 mm of leg cycling or rest across two randomized sessions: mental stress + non-exercise control (MS) and mental stress + exercise (MS + EXE). the Stroop Color-Word Test was used to elicit mental stress. Blood samples were drawn at baseline and following sessions to isolate mononuclear cells. These cells were cultured in fibronectin-coated plates for seven days, and EPCs were identified by immunofluorescence (acLDL(+)/UEA-I Lectin(+)).Key findings: All subjects presented similar increases in mean blood pressure and heart rate during the mental stress test (P < 0.01) in both the MS and MS + EXE sessions. Number of EPCs was not different between groups at baseline in both sessions (P > 0.05). the EPC response to MS and MS + EXE was increased in healthy subjects, whereas it was decreased in subjects with MetS (P < 0.04). in healthy subjects, the EPC response to MS + EXE was greater than the response to MS alone (P = 0.03).Significance: An exercise session increased EPCs in healthy subjects but did not prevent the EPC reduction induced by mental stress among subjects with MetS. (C) 2015 Elsevier Inc. All rights reserved.
Keywords Cardiometabolic risk
Culture
Exercise
Progenitor cells
Stress
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CNPq: 307251/2009-8
FAPERJ: E-26/102.378/2009
CAPES: 2690/09-8
Date 2015-02-15
Published in Life Sciences. Oxford: Pergamon-Elsevier B.V., v. 123, p. 93-99, 2015.
ISSN 0024-3205 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 93-99
Origin http://dx.doi.org/10.1016/j.lfs.2014.12.026
Access rights Closed access
Type Article
Web of Science ID WOS:000350710000012
URI http://repositorio.unifesp.br/handle/11600/38761

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