Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen

Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen

Author Rampazo, Eline V. Google Scholar
Amorim, Kelly N. S. Google Scholar
Yamamoto, Marcio M. Google Scholar
Panatieri, Raquel Hoffmann Google Scholar
Rodrigues, Mauricio Martins Autor UNIFESP Google Scholar
Boscardin, Silvia B. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Natl Inst Sci & Technol Vaccines
Abstract Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. in the present work, we used the mAb alpha DEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (ASP-2) to identify a region of this protein recognized by specific T cells. the hybrid alpha DEC-ASP2 mAb was successfully generated and preserved its ability to bind the DEC205 receptor. Immunization of BALB/c mice with the recombinant mAb in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)) specifically enhanced the number of IFN-gamma producing cells and CD4+ T cell proliferation when compared to mice immunized with a mAb without receptor affinity or with the non-targeted ASP-2 protein. the strong immune response induced in mice immunized with the hybrid alpha DEC-ASP2 mAb allowed us to identify an ASP-2-specific CD4+ T cell epitope recognized by the BALB/c MHCII haplotype. We conclude that targeting parasite antigens to DCs is a useful strategy to enhance T cell mediated immune responses facilitating the identification of new T-cell epitopes.
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
BNP-Paribas Bank
commercial source BNP-Paribas Bank
Grant number CNPq: 15203*12
FAPESP: 2007/08648-9
Date 2015-02-13
Published in Plos One. San Francisco: Public Library Science, v. 10, n. 2, 13 p., 2015.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 13
Origin http://dx.doi.org/10.1371/journal.pone.0117778
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000350682600087
URI http://repositorio.unifesp.br/handle/11600/38759

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