Cecropia pachystachya extract attenuated the renal lesion in 5/6 nephrectomized rats by reducing inflammation and renal arginase activity

Cecropia pachystachya extract attenuated the renal lesion in 5/6 nephrectomized rats by reducing inflammation and renal arginase activity

Author Maquiaveli, Claudia C. Google Scholar
Silva, Edson R. da Google Scholar
Rosa, Lara C. Google Scholar
Francescato, Heloisa D. C. Google Scholar
Lucon Junior, Joao Francisco Google Scholar
Silva, Cleonice G. A. Google Scholar
Casarini, Dulce E. Autor UNIFESP Google Scholar
Ronchi, Fernanda A. Autor UNIFESP Google Scholar
Coimbra, Terezila M. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Ethnopharmacological relevance: the plant Cecropia pachystachya Trecul has been used in Brazilian folk medicine to treat hypertension, bladder and kidney inflammation and renal diseases. the aim of this study was to evaluate the potential of the aqueous fraction from the ethanolic extract of Cecropia pachystachya (FCP) in the management of hypertension, inflammation and progressive renal disease in rats submitted to 5/6 nephrectomy.Materials and methods: Thirty male Wistar rats submitted to 5/6 nephrectomy (5/6 NE) were untreated (NE) or treated (NE+FCP) with the FCP (0.5 g/kg/day). the treatment started 15 days after surgery, and the rats were followed for a period of 60 days. Systolic blood pressure (SBP) and albuminuria were evaluated from 15-60 days after the surgical procedure. Function and estructural renal changes, TGF-beta (transforming growth factor beta), MCP-1 (monocyte chemoattractant protein-1) and nitric oxide (NO) urinary excretion were analyzed. Expression and activity of the renal enzymes arginase (ARG), angiotensin converting enzyme (ACE), and MAP kinase p-JNK expression also were analyzed.Results: the nephrectomized rats developed progressive albuminuria and increased SBP that was less intense in the treated group. There was a reduction in the glomerular filtration rate (GFR) in the nephrectomized rats, which was attenuated by treatment with FCP extract. the treatment with FCP also attenuated the histological changes, reduced the expression and activity of renal arginase, the number of macrophages (ED-1 positive cells) and the p-JNK expression in the renal cortex of the rats submitted to 5/6 NE. the urinary excretion of TGF-beta was less intense in the treated group and was associated with the reduction of the expression and activity of the renal arginase.Conclusions: These results suggest that the reduction of renal arginase activity, p-JNK and TGF-beta expression can explain the mechanism by which the treatment with C. pachystachya reduced the inflammation and improved renal function. This study presents the potential use of Cecropia pachystachya in the treatment of chronic renal diseases. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
Keywords Arginase
Angiotensin II
Renal diseases
Cecropia pachystachya
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 11/50391-0
FAPESP: 12/17059-5
Date 2014-12-02
Published in Journal of Ethnopharmacology. Clare: Elsevier B.V., v. 158, p. 49-57, 2014.
ISSN 0378-8741 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 49-57
Origin http://dx.doi.org/10.1016/j.jep.2014.09.042
Access rights Closed access
Type Article
Web of Science ID WOS:000348084600007
URI http://repositorio.unifesp.br/handle/11600/38542

Show full item record


File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)




My Account