Carnitine partially protects the rat testis against the late damage produced by doxorubicin administered during pre-puberty

Carnitine partially protects the rat testis against the late damage produced by doxorubicin administered during pre-puberty

Author Cabral, R. E. L. Autor UNIFESP Google Scholar
Okada, F. K. Autor UNIFESP Google Scholar
Stumpp, Taiza Autor UNIFESP Google Scholar
Vendramini, V. Autor UNIFESP Google Scholar
Miraglia, Sandra Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Doxorubicin, an anticancer drug, is widely included in chemotherapy protocols to combat childhood cancer. Carnitine, an important quaternary amine, is present in testis and epididymis and is involved in sperm maturation; it has been used in infertility treatment. in a previous study, our group observed that l-carnitine given before etoposide, another chemotherapeutic drug, reduces the spermatogenic damage and protects germ cells against apoptosis. This study aimed to evaluate the antiapoptotic and cytoprotective actions of l-carnitine in long- and mid-term basis, on the seminiferous epithelium of doxorubicin-treated pre-pubertal rats. Forty-eight 30-day-old male Wistar rats were distributed into four groups: sham-control; doxorubicin; carnitine; carnitine/doxorubicin (l-carnitine injected 1h before doxorubicin). the rats were submitted to euthanasia at 64 and 100days of age and their testes were collected for biometric, morphometric, and histopathological analyses. the numerical density of apoptotic germ cells was obtained (TUNEL method). in adult phase (100days), the following spermatic parameters were analyzed: mature spermatid (19 step) count and sperm daily production per testis; sperm number and transit time through the epididymal caput/corpus and cauda; frequency of morphologically abnormal spermatozoa (from epididymal fluid), as well as sperm DNA integrity (Comet assay). the testicular and spermatic parameters at both ages were improved in rats treated with carnitine before doxorubicin. At 64days, the TUNEL-positive germ cell frequency was lower in the carnitine/doxorubicin-treated rats comparatively to the doxorubicin-treated rats. At 100days of age, the sperm DNA fragmentation was also lower in the previously carnitine-treated rats, as evidenced by the analysis of three parameters. Carnitine reduced the late testicular and spermatic damages caused by doxorubicin, probably providing a partial cytoprotection against the deleterious action of doxorubicin administration to pre-pubertal rats. However, further studies shall be undertaken to investigate the protective mechanisms involved in such germ cell preservation.
Keywords carnitine
doxorubicin
germ cells
pre-pubertal
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-11-01
Published in Andrology. Hoboken: Wiley-Blackwell, v. 2, n. 6, p. 931-942, 2014.
ISSN 2047-2919 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 931-942
Origin http://dx.doi.org/10.1111/andr.279
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344328700017
URI http://repositorio.unifesp.br/handle/11600/38362

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