Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression

Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression

Author Varela, Patricia Autor UNIFESP Google Scholar
Escosteguy-Neto, João Carlos Autor UNIFESP Google Scholar
Coelho, Carolina Tesone Autor UNIFESP Google Scholar
Mello, Luiz Eugenio Araujo de Moraes Autor UNIFESP Google Scholar
Silveira, Dartiu Xavier da Autor UNIFESP Google Scholar
Santos-Junior, Jair Guilherme Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fac Ciencias Med São Paulo
Abstract To address the role of mixed anxiety/mood disorder on appetitive associative learning, we verify whether previous chronic light deprivation changes ethanol-induced conditioned place preference and its respective expression of c-Fos and pCREB, markers of neuronal activity and plasticity. the experimental group was maintained in light deprivation for 24 h for a period of 4 wk. Subsequently, it was adapted to a standard light-dark cycle for 1 wk. As a control, some mice were maintained in standard cycle for a period of 4 wk (Naive group). Then, all animals were submitted to behavioral tests to assess emotionality: elevated plus maze; open field; and forced swim. After that, they were submitted to ethanol-induced conditioned place preference. Ninety minutes after the place preference test, they were perfused, and their brains processed for c-Fos and pCREB immunohistochemistry. Light deprivation induced anxiety-like trait (elevated plus maze), despair (forced swim), and hyperlocomotion (open field), common features seen in other animal models of depression. Ethanol-induced conditioned place preference was accompanied by increases on c-Fos and pCREB in the hippocampus, prefrontal cortex and striatum. Interestingly, mice previously submitted to light deprivation did not develop either acquisition and/or expression of ethanol-induced conditioned place preference or increases in c-Fos and pCREB. Therefore, chronic light deprivation mimics several behavioral aspects of other animal models of depression. Furthermore, it could be useful to study the neurochemical mechanisms involved in the dual diagnosis. However, given its likely deleterious effects on appetitive associative memory, it should be used with caution to investigate the cognitive aspects related to the dual diagnosis.
Keywords Anxiety
c-Fos
conditioned place preference
depression
light deprivation
pCREB
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-11-01
Published in International Journal of Neuropsychopharmacology. New York: Cambridge Univ Press, v. 17, n. 11, p. 1815-1830, 2014.
ISSN 1461-1457 (Sherpa/Romeo, impact factor)
Publisher Cambridge Univ Press
Extent 1815-1830
Origin http://dx.doi.org/10.1017/S1461145714000480
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000345005400010
URI http://repositorio.unifesp.br/handle/11600/38355

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