Influence of acute treatment with sibutramine on the sympathetic neurotransmission of the young rat vas deferens

Influence of acute treatment with sibutramine on the sympathetic neurotransmission of the young rat vas deferens

Author Souza, Bruno Palmieri de Autor UNIFESP Google Scholar
Silva Junior, Edilson Dantas da Autor UNIFESP Google Scholar
Jurkiewicz, Aron Autor UNIFESP Google Scholar
Jurkiewicz, Neide Hyppolito Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The effects of acute treatment with sibutramine on the peripheral sympathetic neurotransmission in vas deferens of young rats were still not evaluated. Therefore, we carried out this study in order to verify the effects of acute sibutramine treatment on the neuronal- and exogenous agonist-induced contractions of the young rat vas deferens. Young 45-day-old male Wistar rats were pretreated with sibutramine 6 mg/kg and after 4 h the vas deferens was used for experiment. the acute treatment with sibutramine was able to increase the potency (pD(2)) of noradrenaline and phenylephrine. Moreover, the efficacy (E) of noradrenaline was increased while the efficacy of serotonin and nicotine were decreased. the maximum effect induced by a single concentration of tyramine was diminished in the vas deferens from treated group. Moreover, the leftward shift of the noradrenaline curves promoted by uptake blockers (cocaine and corticosterone) and beta-adrenoceptor antagonist (propranolol) was reduced in the vas deferens of treated group. the initial phasic and secondary tonic components of the neuronal evoked contractions of vas deferens from treated group at the frequencies of 2 Hz were decreased. Moreover, only the initial phasic component at 5 Hz was diminished by the acute treatment with sibutramine. in conclusion, we showed that the acute treatment with sibutramine in young rats was able to affect the peripheral sympathetic nervous system by inhibition of noradrenaline uptake and reduction of the neuronal content of this neurotransmitter, leading to an enhancement of vas deferens sensitivity to noradrenaline. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Sibutramine
Acute treatment
Vas deferens
Young rats
Sympathetic neurotransmission
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-09-05
Published in European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 738, p. 118-124, 2014.
ISSN 0014-2999 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 118-124
Origin http://dx.doi.org/10.1016/j.ejphar.2014.05.035
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000339998000015
URI http://repositorio.unifesp.br/handle/11600/38206

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