Immunosensor for HIV-1 Diagnostics Based on Immobilization of the Antigenic Peptide p24-3 Into Liposomes
Moraes, Marli L. [UNIFESP]
Rodrigues, Valquiria C.
Soares, Juliana C.
Souza, Nara C. de
Oliveira, Osvaldo N.
Is part ofJournal of Nanoscience and Nanotechnology
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In this work we developed an immunosensor for HIV-1 diagnostics that exploits the biorecognition between the antibody anti-p24 and the antigenic peptide p24-3 (AMATLRAEQASQEVKNWMTETL-LVQNA) derived from the HIV-1 p24 protein. p24-3 was encapsulated in phospholipid liposomes and immobilized in layer-by-layer (LbL) films produced with polyethyleneimine (PEI). the incorporation of p24-3 into liposomes was investigated using circular dichroism (CD) spectroscopy, from which an increase in the alpha helix conformation could be noted. the maximum fluorescence emission for p24-3 occurred at 340 nm in solution, compatible with the tryptophan residue being exposed to the solvent, and at 335 and 322 nm when in liposomes and PEI/p24-3-liposome LbL films, respectively. This blue shift is consistent with the tryptophan being in a partially buried environment. With the preserved structure in the LbL films, p24-3 could recognize the anti-p24 antibody in impedance spectroscopy measurements. Therefore, LbL films containing p24-3 may be suitable for detecting HIV-1 in a low-cost, easy-to-use immunosensing assay.
CitationJournal of Nanoscience and Nanotechnology. Valencia: Amer Scientific Publishers, v. 14, n. 9, p. 6638-6645, 2014.
SponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
rede nBioNet (Brazil)
microfabrication laboratory (LMF/LNNano) (Brazil)
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