Functional effects of alcohol withdrawal syndrome on peripheral sympathetic neurotransmission in vas deferens of adult rats

Show simple item record

dc.contributor.author Bomfim, Guilherme Henrique Souza [UNIFESP]
dc.contributor.author Verde, Luciana Ferreira [UNIFESP]
dc.contributor.author Frussa-Filho, Roberto [UNIFESP]
dc.contributor.author Jurkiewicz, Aron [UNIFESP]
dc.contributor.author Jurkiewicz, Neide Hyppolito [UNIFESP]
dc.date.accessioned 2016-01-24T14:37:35Z
dc.date.available 2016-01-24T14:37:35Z
dc.date.issued 2014-07-11
dc.identifier http://dx.doi.org/10.1016/j.lfs.2014.05.002
dc.identifier.citation Life Sciences. Oxford: Pergamon-Elsevier B.V., v. 108, n. 1, p. 34-43, 2014.
dc.identifier.issn 0024-3205
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37988
dc.description.abstract Aims: Alcohol withdrawal syndrome (AWS) is characterized by a set of physiological modifications triggered by abrupt withdrawal and/or decreasing consumption of ethanol (EtOH), which May manifest 16-48 h after ceasing consumption. the relationship between the effects of AWS and central and peripheral sympathetic neurotransmission is unknown. This study investigates the possible mechanisms on the sympathetic system during periods of AWS.Main methods: Male Wistar rats were treated with EtOH (6-10 g/kg/day/v.o. 5 days). Subsequently, 1 h, 24 h, 48 h and 120 h after administration of the last dose of EtOH, the animals were sacrificed, and their vas deferens (VD) were removed to perform the following evaluations: (a) concentration-effect curves of sympathetic agonist; (b) activity of alpha(2)-adrenoreceptor; (c) function of voltage-dependent calcium channels (Cav); and (d) release of endogenous catecholamines measured in real time coupled to HPLC.Key findings: the results showed that the maximum effects of contraction were increased by agonists tested in at 24 h and 48 h EtOH withdrawal. the inhibitory affinity (pIC(50)) of guanfacine was decreased 24 h EtOH withdrawal. the function of Cav was also decreased as pIC50 values dropped 24 h and 48 h EtOH withdrawal. the release of catecholamines increased 48 h after EtOH withdrawal. It is suggested that AWS triggers hyperactivity in peripheral sympathetic neurotransmission.Significance: the mechanisms underlying hyperactivity are possibly explained by a failure of autoregulation from catecholamines released by alpha(2)-adrenoreceptors and/or an increase of Cav function, which may be potential targets to attenuate the symptoms of AWS at the peripheral level. (C) 2014 Published by Elsevier Inc. en
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 34-43
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Life Sciences
dc.rights Acesso restrito
dc.subject Alcohol withdrawal syndrome en
dc.subject Peripheral sympathetic neurotransmission en
dc.subject Catecholamines en
dc.subject Calcium channels en
dc.subject Vas deferens en
dc.title Functional effects of alcohol withdrawal syndrome on peripheral sympathetic neurotransmission in vas deferens of adult rats en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Fed Univ São Paulo Unifesp, Dept Pharmacol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Fed Univ São Paulo Unifesp, Dept Pharmacol, BR-04044020 São Paulo, Brazil
dc.description.sponsorshipID CAPES: 8477/2013-2
dc.description.sponsorshipID FAPESP: 2011/51778-3
dc.identifier.doi 10.1016/j.lfs.2014.05.002
dc.description.source Web of Science
dc.identifier.wos WOS:000338691600006



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account