Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats

Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats

Author Maganhin, Carla C. Autor UNIFESP Google Scholar
Simoes, Ricardo S. Google Scholar
Fuchs, Luiz F. P. Google Scholar
Sasso, Gisela R. S. Autor UNIFESP Google Scholar
Simoes, Manuel J. Autor UNIFESP Google Scholar
Baracat, Edmund C. Google Scholar
Soares, Jose M. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Objective: To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats.Design: Experimental research.Setting: University research laboratory.Animal(s): Thirty female adult rats. Intervention(s): Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 mu g/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary.Main Outcome Measure(s): Biologic molecular study followed by immunohistochemical analysis.Result(s): the changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups.Conclusion(s): Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis. (C)2014 by American Society for Reproductive Medicine.
Keywords Gene expression
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2006/60412-7
FAPESP: 2007/54398-4
Date 2014-07-01
Published in Fertility and Sterility. New York: Elsevier B.V., v. 102, n. 1, p. 291-298, 2014.
ISSN 0015-0282 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 291-298
Access rights Closed access
Type Article
Web of Science ID WOS:000338932300049

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