Ascorbic acid reduces gentamicin-induced nephrotoxicity in rats through the control of reactive oxygen species
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2014-04-01
Autores
Moreira, Miriam A.
Nascimento, Marcos A.
Bozzo, Tatiana A.
Cintra, Alvaro [UNIFESP]
Silva, Sonia M. da [UNIFESP]
Dalboni, Maria Aparecida [UNIFESP]
Mouro, Margaret G.
Higa, Elisa M. S. [UNIFESP]
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Background & aim: Oxidative stress has been implicated in the pathophysiology of many forms of acute renal failure. the aim was examine the effect of vitamin C on oxidative stress and its relationship with nitric oxide on gentamicin-induced nephrotoxicity in rats.Methods: We utilized 32 Wistar rats allocated in four groups of eight animals each: control (CTL), vitamin C (VIT C), gentamicin (GENTA), and GENTA + VIT C; all groups were treated during seven days.Results: Serum urea and creatinine, serum and renal tissue malondialdehyde, blood superoxide anion and hydrogen peroxide in GENTA were increased vs CTL and vs VIT C, and decreased in GENTA + VIT C vs GENTA (all P < 0.05). Serum nitric oxide increased in GENTA vs CTL and vs VIT C, and reduced in GENTA + VII C vs GENTA (P < 0.001). Urinary nitric oxide was reduced in GENTA vs CTL and vs VIT C and increased in GENTA + VIT C vs GENTA (P < 0.001). Severe degeneration of proximal tubules was present in GENTA, but only mild lesions were observed in GENTA + VIT C.Conclusion: This study suggests that VIT C is a valuable tool to protect against GENTA-induced nephrotoxicity, by reducing reactive oxygen species and increasing the nitric oxide. (C) 2013 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
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Clinical Nutrition. Edinburgh: Churchill Livingstone, v. 33, n. 2, p. 296-301, 2014.