Effects of prenatal and lactation nicotine exposure on rat testicular interstitial tissue

Effects of prenatal and lactation nicotine exposure on rat testicular interstitial tissue

Author Paccola, Camila Cicconi Autor UNIFESP Google Scholar
Neves, F. M. O. Autor UNIFESP Google Scholar
Cipriano, I. Autor UNIFESP Google Scholar
Stumpp, Taiza Autor UNIFESP Google Scholar
Miraglia, Sandra Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Nicotine is largely consumed as a component of cigarettes. It induces apoptosis, interferes with endocrine function by changing the sex hormones secretion and leads to male infertility. Testosterone is produced from cholesterol by Leydig cells (LC), with the participation of testicular macrophages (MO). Thus, to investigate whether nicotine administration to pregnant and lactating rats changes cholesterol and sexual hormone levels and LC and MO populations of offspring, female rats received nicotine (2mg/kg/day) through osmotic minipumps from the first day of pregnancy up to the end of weaning. At 1, 30, 60 and 90days post-partum (dpp) the plasma cholesterol and testosterone levels were obtained, as well as the biometric, histopathological and stereological testicular parameters. Nicotine reduced the body weight, cholesterol levels and lipid droplet number in foetal LC at 1dpp. the number of apoptotic LC did not change in the offspring of nicotine group at any age studied. No alterations in the numerical densities of MO and LC occurred at 60 and 90dpp. Hypertrophy of mature LC and increase in cholesterol and testosterone levels were noted at 90dpp. in conclusion, nicotine when administered to rats throughout pregnancy and lactation induces morphofunctional alterations of foetal and mature LC and affects cholesterol and testosterone levels.
Keywords Leydig cell
testicular macrophage
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2009/07855-6
Date 2014-03-01
Published in Andrology. Hoboken: Wiley-Blackwell, v. 2, n. 2, p. 175-185, 2014.
ISSN 2047-2919 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 175-185
Origin http://dx.doi.org/10.1111/j.2047-2927.2013.00168.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000331696100004
URI http://repositorio.unifesp.br/handle/11600/37461

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