Immunoblotting of soluble antigens in Paracoccidioides brasiliensis culture

Date
2014-03-01
Author
Carlos, Nilson de Jesus
Pinto, Debora Alves
Ono, Mario Augusto
Venancio, Emerson Jose
Camargo, Zoilo Pires de [UNIFESP]
Sano, Ayako
Itano, Eiko Nakagawa
Type
Artigo
ISSN
0385-5600
Is part of
Microbiology and Immunology
DOI
10.1111/1348-0421.12137
Metadata
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Abstract
This study investigated the major soluble antigens produced by Paracoccidioides brasiliensis (Pb339) cultured in solid Sabouraud (pH 5.6 and 8.5), Sabouraud plus brain heart infusion and liquid tomato juice-enriched complex medium media at intervals of 3 days over 30 days by immunoblotting and concluded that, to optimize the source of each antigen, both time and growth conditions should be considered.
Citation
Microbiology and Immunology. Hoboken: Wiley-Blackwell, v. 58, n. 3, p. 212-214, 2014.
Keywords
paracoccidioidomycosis
virulence factor
immunodiagnosis
fungi
Sponsorship
Ministry of Education/University Extension Program
Research Program for the Health System(PPSUS)/Araucaria Foundation
State University of Londrina
URI
http://repositorio.unifesp.br/handle/11600/37460
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