ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle

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Data
2014-02-01
Autores
Calderano, Simone
Godoy, Patricia
Soares, Daiane
Sant'Anna, Osvaldo Augusto
Schenkman, Sergio [UNIFESP]
Elias, M. Carolina
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Trypanosoma cruzi alternates between replicative and non-replicative stages. We analyzed the expression of components of the pre-replication machinery TcORC1/CDC6 and TcMCM7 and their interaction with DNA in all T. cruzi stages. TcORC1/CDC6 remains in the nuclear space during all stages of the life cycle and interacts with DNA in the replicative stages; however, it does not bind to DNA in the non-replicative forms. Moreover, TcMCM7 is not present in the non-replicative stages. These data suggest that the lacking of DNA replication during the T. cruzi life cycle may be a consequence of the blocking of TcORC1/CDC6-DNA interaction and of the down regulation of the TcMCM7 expression. (C) 2014 Elsevier B.V. All rights reserved.
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Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 193, n. 2, p. 110-113, 2014.