Association of COX2 gene hypomethylation with intestinal type gastric cancer in samples of patients from northern Brazil

Date
2014-02-01Author
Vieira de Melo, Cynthia Farias
Gigek, Carolina Oliveira [UNIFESP]
Silva, Juarez Nobrega da
Cardoso Smith, Marilia de Arruda [UNIFESP]
Araujo, Rubistenia Miranda de
Burbano, Rommel Rodriguez
Lima, Eleonidas Moura
Type
ArtigoISSN
1010-4283Is part of
Tumor BiologyDOI
10.1007/s13277-013-1148-6Metadata
Show full item recordAbstract
To verify the methylation status of THBS1, GPX3, and COX2 genes and to evaluate their association with Helicobacter pylori in gastric adenocarcinomas. Methylation-sensitive restriction enzyme PCR assay was performed in 16 diffuse type gastric cancer samples, 23 intestinal type, and 15 normal stomach tissue. the presence of H. pylori was performed by amplification of the fragment of the 16S rRNA. Statistical analyses were performed using Fisher's exact test. the hypermethylation of GPX3, THBS1, and COX2 occurred in 18 (n = 7), 5 (n = 2), and 36 % (n = 14) of gastric cancer samples, respectively, whereas in normal samples, it was found in 13, 7, and 67 %. the presence of H. pylori was detected in 67 % of gastric cancer samples and 67 % in normal gastric samples. the methylation of THBS1 and GPX3 was not significantly different between the types of tumors, normal sample, the presence of H. pylori, or clinicopathological variables studied (P > 0.05). However, the methylation status of the gene COX2 is significantly different between normal tissue and intestinal type gastric cancer (P = 0.02). Therefore, our results suggest that the methylation status of the gene COX2 is associated with the intestinal type of gastric cancer.
Citation
Tumor Biology. Dordrecht: Springer, v. 35, n. 2, p. 1107-1111, 2014.Keywords
Gastric cancerMethylation
COX2
Sponsorship
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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