The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion

Nenhuma Miniatura disponível
Data
2014-01-01
Autores
Brunelli, Roberta de Matos
Rodrigues, Natalia Camargo
Ribeiro, Daniel Araki [UNIFESP]
Fernandes, Kelly Rossetti [UNIFESP]
Magri, Angela Maria Paiva [UNIFESP]
Assis, Livia [UNIFESP]
Parizotto, Nivaldo Antonio
Cliquet, Alberto
Renno, Ana Claudia Muniz [UNIFESP]
Abreu, Daniela C. C.
Orientadores
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
The objective of this study was to assess the effects of 780-nm low-level laser therapy at different periods of 7, 14 and 21 days after cryolesion, including the dose (10 or 50 J/cm(2)), to promote a better muscle repair evidenced by histopathological and immunohistochemical analyses. Fifty-four male rats were divided into three groups: injured control group (CG)-injured animals without any treatment; injured 780-nm laser-treated group, at 10 J/cm(2) (G10); and injured 780-nm laser-treated group, at 50 J/cm(2) (G50). Each group was divided into three subgroups (n = 6): 7, 14 and 21 days post-injury. Histopathological findings revealed better organised muscle fibres in the G10 and G50 during the periods of 7 and 14 days compared to the CG. the G10 and G50 during the 7 days showed a significant reduction (p < 0.05) of lesion area compared to the CG, without differences between groups treated for 14 and 21 days. the G10 showed an increase of the amount of vessels after 14 days compared to the G50, but not in relation to controls. With regard to the immunohistochemical analyses of the MyoD factor, the G10 and G50 during the 7 days showed higher concentrations of immunomarkers than controls. Myogenin immunomarkers were similarly observed at days 7 and 14 in all the three groups analysed, whereas immunomarkers were found in none of the groups after 21 days of laser therapy. the results showed that laser, regardless the applied dose, has positive effects on muscle repair.
Descrição
Citação
Lasers in Medical Science. London: Springer London Ltd, v. 29, n. 1, p. 91-96, 2014.
Coleções