L-arginine abolishes the hypothalamic serotonergic activation induced by central interleukin-1 beta administration to normal rats

L-arginine abolishes the hypothalamic serotonergic activation induced by central interleukin-1 beta administration to normal rats

Author Iuras, Anderson Autor UNIFESP Google Scholar
Telles, Monica Marques Autor UNIFESP Google Scholar
Andrade, Iracema Senna de Autor UNIFESP Google Scholar
Santos, Gianni Mara Silva dos Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Silveira, Vera Lucia Flor Autor UNIFESP Google Scholar
Ribeiro, Eliane Beraldi Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract IL-1 beta-induced anorexia may depend on interactions of the cytokine with neuropeptides and neurotransmitters of the central nervous system control of energy balance and serotonin is likely to be one catabolic mediator targeted by IL-1 beta. in the complex interplay involved in feeding modulation, nitric oxide has been ascribed a stimulatory action, which could be of significance in counteracting IL-1 beta effects.The present study aims to explore the participation of the nitric oxide and the serotonin systems on the central mechanisms induced by IL-1 beta and the relevance of their putative interactions to IL-1 beta hypophagia in normal rats. Serotonin levels were determined in microdialysates of the ventromedial hypothalamus after a single intracerebroventricular injection of 10 ng of IL-1 beta, with or without the pre-injection of 20 mu g of the nitric oxide precursor L-arginine. IL-1 beta significantly stimulated hypothalamic serotonin extracellular levels, with a peak variation of 130 +/-37% above baseline. IL-1 beta also reduced the 4-h and the 24-h food intakes (by 23% and 58%, respectively). the IL-1 beta-induced serotonergic activation was abolished by the pre-injection of L-arginine while the hypophagic effect was unaffected.The data showed that one central effect of IL-1 beta is serotonergic stimulation in the ventromedial hypothalamus, an action inhibited by nitric oxide activity. It is suggested that, although serotonin participates in IL-1 beta anorexia, other mechanisms recruited by IL-1 beta in normal rats are able to override the absence of the serotonergic hypophagic influence.
Keywords Brain microdialysis
Food intake
Nitric oxide
Serotonin ventromedial hypothalamus
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2013-12-07
Published in Journal of Neuroinflammation. London: Biomed Central Ltd, v. 10, 7 p., 2013.
ISSN 1742-2094 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 7
Origin https://dx.doi.org/10.1186/1742-2094-10-147
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000329208000001
URI https://repositorio.unifesp.br/handle/11600/37075

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