Evaluation of antimicrobial and antimalarial activities of crude extract, fractions and 4-nerolidylcathecol from the aerial parts of Piper umbellata L. (Piperaceae)

Evaluation of antimicrobial and antimalarial activities of crude extract, fractions and 4-nerolidylcathecol from the aerial parts of Piper umbellata L. (Piperaceae)

Author Bagatela, Bianca Souza Autor UNIFESP Google Scholar
Lopes, Andrey Pereira Autor UNIFESP Google Scholar
Affonso Fonseca, Fernando Luiz Autor UNIFESP Google Scholar
Andreo, Marcio Adriano Autor UNIFESP Google Scholar
Nanayakkara, Dhammika N. P. Google Scholar
Bastos, Jairo Kenupp Google Scholar
Perazzo, Fabio Ferreira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Mississippi
Universidade de São Paulo (USP)
Abstract Presently, natural products, such as Piper umbellata L. (Piperaceae), have been evaluated as sources of antimicrobial agents with efficacies against microorganisms. the in vitro antimicrobial activity was performed by broth micro-dilution susceptibility assay, according to the protocols of the National Committee for Clinical Laboratory Standards, and described the antibacterial and antifungal activities of crude ethanolic extract and fractions obtained by partitions sequentially among water-methanol, methylene chloride and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol from the aerial parts of P. umbellata L. Amphotericin B and ciprofloxacin were used as controls. Among the microorganism cultures, hydromethanol fraction demonstrated the pre-eminent antifungal activity. 4-Nerolidylcathecol was the only tested plant component that exhibited activity against all the selected microorganisms, suggesting its great potential as a source for the development of new drugs. in order to estimate the antimalarial activity of P. umbellata L., a micro-dilution method protocol, parasite lactate dehydrogenase assay, with a Plasmodium falciparum Sierra Leone (D6) clone was utilised. the antimalarial agent artemisinin was used as control. 4-Nerolidylcathecol exhibited the best antimalarial activity; however, it was not significant when compared with control. These in vitro results do not justify the use of P. umbellata L. in malaria patients. However, there is a possibility of 4-nerolidylcathecol, after biotransformation, exhibiting a significant antimalarial activity in in vivo assays. However, 4-nerolidylcathecol demonstrated to possess a broad antimicrobial activity which is, in fact, a promising source for the development of new therapeutic agents.
Keywords Piperaceae
Piper umbellata L
antimicrobial activity
antimalarial activity
in vitro
4-nerolidylcathecol
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CAPES: CBE/PDEE 00012/2003
CAPES: BEX 2824/03-5
Date 2013-12-01
Published in Natural Product Research. Abingdon: Taylor & Francis Ltd, v. 27, n. 23, p. 2202-2209, 2013.
ISSN 1478-6419 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Ltd
Extent 2202-2209
Origin http://dx.doi.org/10.1080/14786419.2013.821123
Access rights Closed access
Type Article
Web of Science ID WOS:000327469000008
URI http://repositorio.unifesp.br/handle/11600/37054

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