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CD11b+GR1+Myeloid Cells Secrete NGF and Promote Trigeminal Ganglion Neurite Growth: Implications for Corneal Nerve Regeneration

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Date
2013-09-01
Author
Sarkar, Joy
Chaudhary, Shweta
Jassim, Sarmad H.
Ozturk, Okan
Chamon, Wallace [UNIFESP]
Ganesh, Balaji
Tibrewal, Sapna
Gandhi, Sonal
Byun, Yong-Soo
Hallak, Joelle
Mahmud, Dolores L.
Mahmud, Nadim
Rondelli, Damiano
Jain, Sandeep
Type
Artigo
ISSN
0146-0404
Is part of
Investigative Ophthalmology & Visual Science
DOI
10.1167/iovs.13-12237
Metadata
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Abstract
PURPOSE. We characterized fluorescent bone marrow cells (YFP+ BMCs) in the thy1-YFP mouse and determine if they promote trigeminal ganglion (TG) cell neurite growth.METHODS. Excimer laser annular keratectomy was performed in thy1-YFP mice, and corneas were imaged. BMCs were harvested from femur and tibia, and the expression of surface markers on YFP+ BMCs was analyzed by flow cytometry. the immunosuppressive action of BMCs (YFP+ and YFP-) was evaluated in an allogenic mixed lymphocyte reaction (MLR). Neurotrophic action of BMCs (YFP+ and YFP-) was determined in compartmental and transwell cultures of dissociated TG cells.RESULTS. Following annular keratectomy, YFP+ BMCs infiltrated the cornea. YFP+ BMCs shared surface markers (CD11b+Gr1+Ly6C+Ly6G-F4/80(low)) with monocytic myeloid-derived suppressor cells (MDSCs), had similar morphology, and suppressed T-cell proliferation in allogenic MLR in a dose-dependent manner. YFP+ BMCs, but not YFP- BMCs, significantly increased growth of TG neurites in vitro. When cultured in a transwell with TG neurites, YFP+ BMCs expressed neurotrophins and secreted nerve growth factor (NGF) in conditioned medium. YFP+ BMCs that infiltrated the cornea maintained their phenotype and actions (neuronal and immune).CONCLUSIONS. YFP+ BMCs in thy1-YFP mice have immunophenotypic features of MDSCs. They secrete NGF and promote neuroregeneration. Their immunosuppressive and neurotrophic actions are preserved after corneal infiltration. These findings increase our understanding of the beneficial roles played by leukocyte trafficking in the cornea and may lead to therapeutic strategies that use NGF-secreting myeloid cells to repair diseased or injured neurons.
Citation
Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 54, n. 9, p. 5920-5936, 2013.
Keywords
cornea
myeloid cell
inflammation
nerve regeneration
Sponsorship
National Eye Institute (NEI)
NEI
Research to Prevent Blindness
Midwest Eye Banks
URI
http://repositorio.unifesp.br/handle/11600/36681
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  • EPM - Artigos [17701]

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