CD11b+GR1+Myeloid Cells Secrete NGF and Promote Trigeminal Ganglion Neurite Growth: Implications for Corneal Nerve Regeneration

CD11b+GR1+Myeloid Cells Secrete NGF and Promote Trigeminal Ganglion Neurite Growth: Implications for Corneal Nerve Regeneration

Author Sarkar, Joy Google Scholar
Chaudhary, Shweta Google Scholar
Jassim, Sarmad H. Google Scholar
Ozturk, Okan Google Scholar
Chamon, Wallace Autor UNIFESP Google Scholar
Ganesh, Balaji Google Scholar
Tibrewal, Sapna Google Scholar
Gandhi, Sonal Google Scholar
Byun, Yong-Soo Google Scholar
Hallak, Joelle Google Scholar
Mahmud, Dolores L. Google Scholar
Mahmud, Nadim Google Scholar
Rondelli, Damiano Google Scholar
Jain, Sandeep Google Scholar
Institution Univ Illinois
Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE. We characterized fluorescent bone marrow cells (YFP+ BMCs) in the thy1-YFP mouse and determine if they promote trigeminal ganglion (TG) cell neurite growth.METHODS. Excimer laser annular keratectomy was performed in thy1-YFP mice, and corneas were imaged. BMCs were harvested from femur and tibia, and the expression of surface markers on YFP+ BMCs was analyzed by flow cytometry. the immunosuppressive action of BMCs (YFP+ and YFP-) was evaluated in an allogenic mixed lymphocyte reaction (MLR). Neurotrophic action of BMCs (YFP+ and YFP-) was determined in compartmental and transwell cultures of dissociated TG cells.RESULTS. Following annular keratectomy, YFP+ BMCs infiltrated the cornea. YFP+ BMCs shared surface markers (CD11b+Gr1+Ly6C+Ly6G-F4/80(low)) with monocytic myeloid-derived suppressor cells (MDSCs), had similar morphology, and suppressed T-cell proliferation in allogenic MLR in a dose-dependent manner. YFP+ BMCs, but not YFP- BMCs, significantly increased growth of TG neurites in vitro. When cultured in a transwell with TG neurites, YFP+ BMCs expressed neurotrophins and secreted nerve growth factor (NGF) in conditioned medium. YFP+ BMCs that infiltrated the cornea maintained their phenotype and actions (neuronal and immune).CONCLUSIONS. YFP+ BMCs in thy1-YFP mice have immunophenotypic features of MDSCs. They secrete NGF and promote neuroregeneration. Their immunosuppressive and neurotrophic actions are preserved after corneal infiltration. These findings increase our understanding of the beneficial roles played by leukocyte trafficking in the cornea and may lead to therapeutic strategies that use NGF-secreting myeloid cells to repair diseased or injured neurons.
Keywords cornea
myeloid cell
inflammation
nerve regeneration
Language English
Sponsor National Eye Institute (NEI)
NEI
Research to Prevent Blindness
Midwest Eye Banks
Grant number National Eye Institute (NEI): K08EY018874
National Eye Institute (NEI): R01EY023656
NEI: EY001792
Date 2013-09-01
Published in Investigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 54, n. 9, p. 5920-5936, 2013.
ISSN 0146-0404 (Sherpa/Romeo, impact factor)
Publisher Assoc Research Vision Ophthalmology Inc
Extent 5920-5936
Origin http://dx.doi.org/10.1167/iovs.13-12237
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000325169500001
URI http://repositorio.unifesp.br/handle/11600/36681

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