Corneal Absorption of a New Riboflavin-Nanostructured System for Transepithelial Collagen Cross-Linking

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dc.contributor.author Bottos, Katia M. [UNIFESP]
dc.contributor.author Oliveira, Anselmo G.
dc.contributor.author Bersanetti, Patricia Alessandra [UNIFESP]
dc.contributor.author Nogueira, Regina F. [UNIFESP]
dc.contributor.author Lima Filho, Acácio Alves de Souza [UNIFESP]
dc.contributor.author Cardillo, José A. [UNIFESP]
dc.contributor.author Schor, Paulo [UNIFESP]
dc.contributor.author Chamon, Wallace [UNIFESP]
dc.date.accessioned 2016-01-24T14:31:53Z
dc.date.available 2016-01-24T14:31:53Z
dc.date.issued 2013-06-13
dc.identifier http://dx.doi.org/10.1371/journal.pone.0066408
dc.identifier.citation Plos One. San Francisco: Public Library Science, v. 8, n. 6, 10 p., 2013.
dc.identifier.issn 1932-6203
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36425
dc.description.abstract Corneal collagen cross-linking (CXL) has been described as a promising therapy for keratoconus. According to standard CXL protocol, epithelium should be debrided before treatment to allow penetration of riboflavin into the corneal stroma. However, removal of the epithelium can increase procedure risks. in this study we aim to evaluate stromal penetration of a biocompatible riboflavin-based nanoemulsion system (riboflavin-5-phosphate and riboflavin-base) in rabbit corneas with intact epithelium. Two riboflavin nanoemulsions were developed. Transmittance and absorption coefficient were measured on corneas with intact epithelia after 30, 60, 120, 180, and 240 minutes following exposure to either the nanoemulsions or standard 0.1% or 1% riboflavin-dextran solutions. for the nanoemulsions, the epithelium was removed after measurements to assure that the riboflavin had passed through the hydrophobic epithelium and retained within the stroma. Results were compared to de-epithelialized corneas exposed to 0.1% riboflavin solution and to the same riboflavin nanoemulsions for 30 minutes (standard protocol). Mean transmittance and absorption measured in epithelialized corneas receiving the standard 0.1% riboflavin solution did not reach the levels found on the debrided corneas using the standard technique. Neither increasing the time of exposure nor the concentration of the riboflavin solution from 0.1% to 1% improved riboflavin penetration through the epithelium. When using riboflavin-5-phosphate nanoemulsion for 240 minutes, we found no difference between the mean absorption coefficients to the standard cross-linking protocol (p = 0.54). Riboflavin nanoemulsion was able to penetrate the corneal epithelium, achieving, after 240 minutes, greater stromal concentration when compared to debrided corneas with the standard protocol (p = 0.002). the riboflavin-5-phosphate nanoemulsion diffused better into the stroma than the riboflavin-base nanoemulsion. en
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 10
dc.language.iso eng
dc.publisher Public Library Science
dc.relation.ispartof Plos One
dc.rights Acesso aberto
dc.title Corneal Absorption of a New Riboflavin-Nanostructured System for Transepithelial Collagen Cross-Linking en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution São Paulo State Univ
dc.description.affiliation Fed Univ São Paulo UNIFESP, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliation São Paulo State Univ, UNESP, Sch Pharmaceut Sci, Dept Pharmaceut, Araraquara, SP, Brazil
dc.description.affiliationUnifesp Fed Univ São Paulo UNIFESP, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.sponsorshipID CAPES: BEX 1325-09-4
dc.identifier.file WOS000321038800025.pdf
dc.identifier.doi 10.1371/journal.pone.0066408
dc.description.source Web of Science
dc.identifier.wos WOS:000321038800025



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