Corneal Absorption of a New Riboflavin-Nanostructured System for Transepithelial Collagen Cross-Linking

Corneal Absorption of a New Riboflavin-Nanostructured System for Transepithelial Collagen Cross-Linking

Author Bottos, Katia M. Autor UNIFESP Google Scholar
Oliveira, Anselmo G. Google Scholar
Bersanetti, Patricia Alessandra Autor UNIFESP Google Scholar
Nogueira, Regina F. Autor UNIFESP Google Scholar
Lima Filho, Acácio Alves de Souza Autor UNIFESP Google Scholar
Cardillo, José A. Autor UNIFESP Google Scholar
Schor, Paulo Autor UNIFESP Google Scholar
Chamon, Wallace Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
São Paulo State Univ
Abstract Corneal collagen cross-linking (CXL) has been described as a promising therapy for keratoconus. According to standard CXL protocol, epithelium should be debrided before treatment to allow penetration of riboflavin into the corneal stroma. However, removal of the epithelium can increase procedure risks. in this study we aim to evaluate stromal penetration of a biocompatible riboflavin-based nanoemulsion system (riboflavin-5-phosphate and riboflavin-base) in rabbit corneas with intact epithelium. Two riboflavin nanoemulsions were developed. Transmittance and absorption coefficient were measured on corneas with intact epithelia after 30, 60, 120, 180, and 240 minutes following exposure to either the nanoemulsions or standard 0.1% or 1% riboflavin-dextran solutions. for the nanoemulsions, the epithelium was removed after measurements to assure that the riboflavin had passed through the hydrophobic epithelium and retained within the stroma. Results were compared to de-epithelialized corneas exposed to 0.1% riboflavin solution and to the same riboflavin nanoemulsions for 30 minutes (standard protocol). Mean transmittance and absorption measured in epithelialized corneas receiving the standard 0.1% riboflavin solution did not reach the levels found on the debrided corneas using the standard technique. Neither increasing the time of exposure nor the concentration of the riboflavin solution from 0.1% to 1% improved riboflavin penetration through the epithelium. When using riboflavin-5-phosphate nanoemulsion for 240 minutes, we found no difference between the mean absorption coefficients to the standard cross-linking protocol (p = 0.54). Riboflavin nanoemulsion was able to penetrate the corneal epithelium, achieving, after 240 minutes, greater stromal concentration when compared to debrided corneas with the standard protocol (p = 0.002). the riboflavin-5-phosphate nanoemulsion diffused better into the stroma than the riboflavin-base nanoemulsion.
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CAPES: BEX 1325-09-4
Date 2013-06-13
Published in Plos One. San Francisco: Public Library Science, v. 8, n. 6, 10 p., 2013.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 10
Origin http://dx.doi.org/10.1371/journal.pone.0066408
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000321038800025
URI http://repositorio.unifesp.br/handle/11600/36425

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