Pulmonary impact of N-acetylcysteine in a controlled hemorrhagic shock model in rats

Pulmonary impact of N-acetylcysteine in a controlled hemorrhagic shock model in rats

Author Saad, Karen Ruggeri Autor UNIFESP Google Scholar
Saad, Paulo Fernandes Autor UNIFESP Google Scholar
Dantas Filho, Luiz Autor UNIFESP Google Scholar
Brito, Jose Mara de Google Scholar
Koike, Marcia Kiyomi Google Scholar
Zanoni, Fernando Luiz Google Scholar
Dolhnikoff, Marisa Google Scholar
Souza Montero, Edna Frasson de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Background: Experimental hemorrhagic shock (HS) is based on controlling bleeding and the treatment of fluid resuscitation to restore tissue oxygenation and perfusion. the HS could promote ischemia/reperfusion injury, which induces a general exacerbation of the inflammatory process, initially compromising the lungs. N-acetylcysteine (NAC), an antioxidant, may attenuate ischemia/reperfusion injury. This study evaluated the effect of NAC in association with fluid resuscitation on pulmonary injury in a controlled HS model in rats.Methods: Male Wistar rats were submitted to controlled HS (mean arterial pressure of 35 mm Hg for 60 min). Two groups were constituted according to resuscitation solution administered: RLG (Ringer's lactate solution) and RLG+NAC (Ringer's lactate in association with 150 mg/kg NAC. A control group was submitted to catheterization only. After 120 min of resuscitation, bronchoalveolar lavage was performed to assess intra-alveolar cell infiltration and pulmonary tissue was collected for assessment of malondialdehyde, interleukin 6, and interleukin 10 and histopathology.Results: Compared with the RLG group, the RLG+NAC group showed lower bronchoalveolar lavage inflammatory cell numbers, lower interstitial inflammatory infiltration in pulmonary parenchyma, and lower malondialdehyde concentration. However, tissue cytokine (interleukin 6 and interleukin 10) expression levels were similar.Conclusion: N-acetylcysteine was associated with fluid resuscitationeattenuated oxidative stress and inflammatory cell infiltration in pulmonary parenchyma. N-acetylcysteine did not modify cytokine expression. (C) 2013 Elsevier Inc. All rights reserved.
Keywords Hemorrhagic shock
Acetylcysteine
Lung
Oxidative stress
Cytokines
Language English
Date 2013-06-01
Published in Journal of Surgical Research. San Diego: Academic Press Inc Elsevier Science, v. 182, n. 1, p. 108-115, 2013.
ISSN 0022-4804 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 108-115
Origin http://dx.doi.org/10.1016/j.jss.2012.07.037
Access rights Closed access
Type Article
Web of Science ID WOS:000318616500024
URI http://repositorio.unifesp.br/handle/11600/36395

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