Antithrombin stabilisation by sulfated carbohydrates correlates with anticoagulant activity

Date
2013-05-01
Author
Lima, Marcelo A. [UNIFESP]
Hughes, Ashley J.
Veraldi, Noemi
Rudd, Timothy R.
Hussain, Rohanah
Brito, Adriana S. [UNIFESP]
Chavante, Suely F.
Tersariol, Ivarne I. [UNIFESP]
Siligardi, Giuliano
Nader, Helena B. [UNIFESP]
Yates, Edwin A.
Type
Artigo
ISSN
2040-2503
Is part of
Medchemcomm
DOI
10.1039/c3md00048f
Metadata
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Abstract
Thermal stabilisation of native antithrombin-III (AT), determined using differential scanning fluorimetry, correlated with the anticoagulant activity of heparin and heparin-related saccharides. Similar conformational changes were induced in native AT by a variety of active and inactive heparin-related sulfated carbohydrates, measured in solution using synchrotron radiation circular dichroism, and their anticoagulant activities. Measurement of native AT stabilisation provides a convenient assay for prospective anticoagulants and represents an additional parameter by which to compare biosimilar heparins.
Citation
Medchemcomm. Cambridge: Royal Soc Chemistry, v. 4, n. 5, p. 870-873, 2013.
Sponsorship
National Science Foundation's National High Magnetic Field Laboratory User Program in the Advanced Magnetic Resonance Imaging and Spectroscopy (AMRIS) Facility (McKnight Brain Institute, University of Florida, USA)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
URI
http://repositorio.unifesp.br/handle/11600/36311
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