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dc.contributor.authorCoulson-Thomas, Vivien Jane [UNIFESP]
dc.contributor.authorCoulson-Thomas, Yvette May [UNIFESP]
dc.contributor.authorGesteira, Tarsis Ferreira [UNIFESP]
dc.contributor.authorPaula, Claudia Alessandra Andrade de [UNIFESP]
dc.contributor.authorCarneiro, Celia Regina Whitaker [UNIFESP]
dc.contributor.authorOrtiz, Valdemar [UNIFESP]
dc.contributor.authorToma, Leny [UNIFESP]
dc.contributor.authorKao, Winston W. Y.
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.date.accessioned2016-01-24T14:31:35Z
dc.date.available2016-01-24T14:31:35Z
dc.date.issued2013-04-15
dc.identifierhttps://dx.doi.org/10.1016/j.yexcr.2013.01.023
dc.identifier.citationExperimental Cell Research. San Diego: Elsevier Inc, v. 319, n. 7, p. 967-981, 2013.
dc.identifier.issn0014-4827
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/36214
dc.description.abstractThe stromal reaction surrounding tumors leads to the formation of a tumor-specific microenvironment, which may play either a restrictive role or a supportive role in the growth and progression of the tumors. Lumican, a small leucine-rich proteoglycan (SLRP) of the extracellular matrix (ECM), regulates collagen fibrillogenesis. Recently, lumican has also been shown to regulate cell behavior during embryonic development, tissue repair and tumor progression. the role of lumican in cancer varies according to the type of tumor. in this study we analyze the role of lumican in the pathogenesis of prostate cancer both in vivo and in vitro. Overall lumican up-regulation was observed in the primary tumors analyzed through both real-time PCR and immunostaining. the increase in lumican expression was observed in the reactive stroma surrounding prostate primary tumors with fibrotic deposition surrounding the acinar glands. in vitro analysis demonstrated that lumican inhibited both the migration and invasion of metastatic prostate cancer cells isolated from lymph node, bone and brain. Moreover, prostate cancer cells seeded on lumican presented a decrease in the formation of cellular projections, lamellipodia detected by a decreased rearrangement in ZO-1, keratin 8/18, integrin beta 1 and MT1-MMP, and invadopodia detected by disruption of alpha-smooth muscle actin, cortactin and N-WASP. Moreover, a significant increase in prostate cancer cell invasion was observed through the peritoneum of lumican knockout mice, further demonstrating the restrictive role lumican present in the ECM has on prostate cancer invasion. in conclusion, lumican present in the reactive stroma surrounding prostate primary tumors plays a restrictive role on cancer progression, and we therefore postulate that lumican could be a valuable marker in prostate cancer staging. (C) 2013 Elsevier Inc. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipNIH/NEI
dc.format.extent967-981
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Cell Research
dc.rightsAcesso restrito
dc.subjectLumicanen
dc.subjectDesmoplasiaen
dc.subjectTumor markersen
dc.subjectProstate canceren
dc.subjectCytoskeletonen
dc.subjectCell migrationen
dc.titleLumican expression, localization and antitumor activity in prostate canceren
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Cincinnati
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Cincinnati, Edith J Crawley Vis Res Ctr, Coll Med, Dept Ophthalmol, Cincinnati, OH 45267 USA
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Urol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Urol, BR-04044020 São Paulo, Brazil
dc.description.sponsorshipIDFAPESP: 2007/59801-1pt
dc.description.sponsorshipIDFAPESP: 2010/52426-3pt
dc.description.sponsorshipIDNIH/NEI: EY 011845
dc.identifier.doi10.1016/j.yexcr.2013.01.023
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000316830100004


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