Ultra-low-molecular-weight heparins: Precise structural features impacting specific anticoagulant activities

Date
2013-03-01Author
Lima, Marcelo A. [UNIFESP]
Viskov, Christian
Herman, Frederic
Gray, Angel L.
Farias, Eduardo H. C. de [UNIFESP]
Cavalheiro, Renan P. [UNIFESP]
Sassaki, Guilherme L.
Hoppensteadt, Debra
Fareed, Jawed
Nader, Helena B. [UNIFESP]
Type
ArtigoISSN
0340-6245Is part of
Thrombosis and HaemostasisDOI
10.1160/TH12-11-0795Metadata
Show full item recordAbstract
Ultra-low-molecular-weight heparins (ULMWHs) with better efficacy and safety ratios are under development; however, there are few structural data available. the main structural features and molecular weight of ULMWHs were studied and compared to enoxaparin. Their monosaccharide composition and average molecular weights were determined and preparations studied by nuclear magnetic resonance spectroscopy, scanning ultraviolet spectroscopy, circular dichroism and gel permeation chromatography. in general, ULMWHs presented higher 3-O-sulphated glucosamine and unsaturated uronic acid residues, the latter being comparable with their higher degree of depolymerisation. the analysis showed that ULMWHs are structurally related to LMWHs; however, their monosaccharide/oligosaccharide compositions and average molecular weights differed considerably explaining their different anticoagulant activities. the results relate structural features to activity, assisting the development of new and improved therapeutic agents, based on depolymerised heparin, for the prophylaxis and treatment of thrombotic disorders.
Citation
Thrombosis and Haemostasis. Stuttgart: Schattauer Gmbh-verlag Medizin Naturwissenschaften, v. 109, n. 3, p. 471-478, 2013.Sponsorship
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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