Characterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patients

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dc.contributor.author Ozcan, Sureyya
dc.contributor.author An, Hyun Joo
dc.contributor.author Vieira, Ana C. [UNIFESP]
dc.contributor.author Park, Gun Wook
dc.contributor.author Kim, Jae Han
dc.contributor.author Mannis, Mark J.
dc.contributor.author Lebrilla, Carlito B.
dc.date.accessioned 2016-01-24T14:31:21Z
dc.date.available 2016-01-24T14:31:21Z
dc.date.issued 2013-03-01
dc.identifier http://dx.doi.org/10.1021/pr3008013
dc.identifier.citation Journal of Proteome Research. Washington: Amer Chemical Soc, v. 12, n. 3, p. 1090-1100, 2013.
dc.identifier.issn 1535-3893
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36043
dc.description.abstract O-Glycans in saliva and tear isolated from patients suffering from ocular rosacea, a form of inflammatory ocular surface disease, were profiled, and their structures were elucidated using high resolution mass spectrometry. We have previously shown that certain structures, particularly sulfated oligosaccharides, increased in the tear and saliva of rosacea patients in this study, the structures of these glycans were elucidated using primarily tandem mass spectrometry. There were important similarities in the glycan profiles of tears and saliva with the majority of the structures in common. the structures of the most abundant species common to both tear and saliva, which were also the most abundant species in both, were elucidated. for sulfated species, the positions of the sulfate groups were localized. the majority of the structures were new, with the sulfated glycans comprising mucin core 1- and core 2-type structures. As both saliva and tear are rich in mucins, it is suggested that the O-glycans are mainly components of mucins. the study further illustrates the strong correspondence between the glycans in the tear and saliva of ocular rosacea patients. en
dc.description.sponsorship National Institutes of Health
dc.description.sponsorship Converging Research Center Program through the Ministry of Education, Science and Technology
dc.format.extent 1090-1100
dc.language.iso eng
dc.publisher Amer Chemical Soc
dc.relation.ispartof Journal of Proteome Research
dc.rights Acesso restrito
dc.subject O-glycan en
dc.subject saliva en
dc.subject tear en
dc.subject mass spectrometry en
dc.subject structural characterization en
dc.subject sulfated O-glycan en
dc.subject sialylated O-glycan en
dc.title Characterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patients en
dc.type Artigo
dc.contributor.institution Univ Calif Davis
dc.contributor.institution Chungnam Natl Univ
dc.contributor.institution Univ Calif
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
dc.description.affiliation Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USA
dc.description.affiliation Chungnam Natl Univ, Grad Sch Analyt Sci & Technol, Taejon 305764, South Korea
dc.description.affiliation Chungnam Natl Univ, Dept Food Nutr, Taejon 305764, South Korea
dc.description.affiliation Univ Calif, Davis Hlth Syst Eye Ctr, Dept Ophthalmol & Vis Sci, Sacramento, CA 95817 USA
dc.description.affiliation Universidade Federal de São Paulo, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Ophthalmol, São Paulo, Brazil
dc.description.sponsorshipID National Institutes of Health: RO1GM049077
dc.description.sponsorshipID Converging Research Center Program through the Ministry of Education, Science and Technology: 2011K000968
dc.identifier.doi 10.1021/pr3008013
dc.description.source Web of Science
dc.identifier.wos WOS:000315708000003



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