Evaluation of FAS and caspase-3 in the endometrial tissue of patients with idiopathic infertility and recurrent pregnancy loss

Evaluation of FAS and caspase-3 in the endometrial tissue of patients with idiopathic infertility and recurrent pregnancy loss

Author Panzan, Michele Quaranta Autor UNIFESP Google Scholar
Mattar, Rosiane Autor UNIFESP Google Scholar
Maganhin, Carla Cristina Autor UNIFESP Google Scholar
Simões, Ricardo dos Santos Autor UNIFESP Google Scholar
Rossi, Alexandre Guilherme Zabeu Autor UNIFESP Google Scholar
Motta, Eduardo Leme Alves da Autor UNIFESP Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Soares Júnior, José Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Objective: To investigate expression of BcL-2, FAS, FAS ligand (FASL) and cleaved caspase-3 in the endometrial tissue of women with idiopathic infertility (with two consecutive failed cycles of in vitro fertilization) and women with idiopathic recurrent pregnancy loss. the control group consisted of fertile women.Study design: Endometrial tissue samples from fertile women (n = 25), women with idiopathic infertility (n = 25) and women with idiopathic recurrent pregnancy loss (n = 25) were collected on the seventh or eighth postovulatory day of their menstrual cycles for evaluation. Expression of BcL-2, FAS, FASL and cleaved caspase-3 was assessed using immunohistochemical methods.Results: Expression of BcL-2 and FAS was significantly higher and lower, respectively, in the women with idiopathic infertility than in the other groups (p < 0.01). Expression of cleaved caspase-3 was significantly lower in the women with idiopathic infertility than in the other groups (p < 0.01). Expression of FASL was similar in all three groups.Conclusion: Disturbances in endometrial apoptosis may be a contributing factor in patients with idiopathic infertility and recurrent pregnancy loss. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
Keywords Endometrium
FAS-FAS-L
BcL-2
Cleaved caspase-3
Idiopathic infertility
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2004/01424-0
Date 2013-03-01
Published in European Journal of Obstetrics & Gynecology and Reproductive Biology. Amsterdam: Elsevier B.V., v. 167, n. 1, p. 47-52, 2013.
ISSN 0301-2115 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 47-52
Origin http://dx.doi.org/10.1016/j.ejogrb.2012.10.021
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000316429200009
URI http://repositorio.unifesp.br/handle/11600/35992

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