Biochemical Analysis and Decomposition Products of Indocyanine Green in Relation to Solvents, Dye Concentrations and Laser Exposure

Biochemical Analysis and Decomposition Products of Indocyanine Green in Relation to Solvents, Dye Concentrations and Laser Exposure

Author Penha, Fernando M. Autor UNIFESP Google Scholar
Rodrigues, Eduardo B. Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Meyer, Carsten H. Google Scholar
Fiod Cost, Elaine de Paula Autor UNIFESP Google Scholar
Dib, Eduardo Autor UNIFESP Google Scholar
Bechara, Etelvino Google Scholar
Lourenco, Andreia Autor UNIFESP Google Scholar
Lima Filho, Acacio A. S. Autor UNIFESP Google Scholar
Freymueller, Edna H. Autor UNIFESP Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Int Chromovitrectomy Collaboration Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Univ Bonn
Abstract Purpose: To investigate pH, ions, osmolarity and precipitation of indocyanine green (ICG), as well as the profile of ICG decomposition products (DPs) after laser exposure and the interaction with quenchers. Methods: ICG was diluted in water, 5% glucose (GL) or balanced salt solution (BSS) to achieve concentrations of 2.5, 1, 0.25 and 0.1 mg/ml. Osmolarity, pH and precipitation were analyzed immediately and after 24 h. Precipitation analyses were done with a scanning electron microscope. Anion and iodate analyses of ICG and infracyanine green (IfCG) were performed by capillary zone electrophoresis. With regard to DPs, 0.5 mg/ml of ICG was assessed with high-performance liquid chromatography (HPLC) after 810-nm laser irradiation. DP profiles were evaluated with ICG dilution in quenchers (Trolox, histidine and DABCO) in 3 concentrations (0.1, 1 and 10 m). Results: BSS promoted iso-osmotic ICG solutions of 208 mOsm (147-266) compared to GL with 177 mOsm. BSS solutions had a higher physiological pH of 7.2 compared with the GL one of 6.55. ICG precipitated more when diluted with BSS (5.95 mg); in contrast, GL showed less precipitate (3.6 mg). IfCG has no iodine derivates and other ICGs have an average 4.6% of iodate derivates. From HPLC analysis, 5 DPs were observed. the rate of DPs was higher when BSS was used (p < 0.05). Five DPs have been generated with ICG, and they may be altered with the quenchers DABCO, histidine and Trolox. Conclusions: BSS dilution induces more precipitation and DPs. ICG dilution in any solvent induces DPs. Quencher use reduces the amount of toxic DPs. (C) 2013 S. Karger AG, Basel
Keywords Indocyanine green
lnfracyanine green
Biochemical analysis
Decomposition products
Internal limiting membrane
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Pan-American Association of Ophthalmology (PAAO)
Date 2013-01-01
Published in Ophthalmologica. Basel: Karger, v. 230, p. 59-67, 2013.
ISSN 0030-3755 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 59-67
Access rights Closed access
Type Article
Web of Science ID WOS:000324444600009

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