Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa

Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa

Author Silva, Erick J. R. Autor UNIFESP Google Scholar
Patrao, Marilia T. C. C. Google Scholar
Tsuruta, James K. Google Scholar
O'Rand, Michael G. Google Scholar
Avellar, Maria Christina W. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ N Carolina
Abstract EPPIN (epididymal protease inhibitor; SPINLW1), an antimicrobial cysteine-rich protein containing both Kunitz and whey acidic protein (WAP)-type four disulfide core protease inhibitor consensus sequences, is a target for male contraception because of its critical role in sperm motility. Here, we characterized EPPIN's expression and cellular distribution in rat tissues and its in vivo regulation by androgens in the epididymis. EPPIN (mRNA and protein) was abundantly expressed in the rat testis and epididymis; we also found that the vas deferens, seminal vesicles, and brain were novel sites of EPPIN expression. PCR studies demonstrated that in addition to Sertoli cells, spermatogenic cells expressed Eppin mRNA. EPPIN was immunolocalized in Sertoli cells and spermatogenic cells (pachytene spermatocytes and round and elongated spermatids) and in epithelial cells and spermatozoa from efferent ductules and epididymis. EPPIN staining was observed on the middle and principal pieces of the flagellum of testicular spermatozoa. Epididymal spermatozoa had more intense EPPIN staining on the flagellum, and the EPPIN staining became apparent on the head and neck regions. This suggested that the EPPIN found on maturing spermatozoa was secreted primarily by the epithelial cells of the epididymis. Surgical castration down-regulated EPPIN expression levels (mRNA and protein) in the caput and cauda epididymis, an effect reversed by testosterone replacement. Altogether, our data suggested that EPPIN expression in rats is more widespread than in humans and mice, and is androgen-dependent in the epididymis. This species could be used as an experimental model to further study EPPIN's role in male fertility. Mol. Reprod. Dev. 79: 832842, 2012. (C) 2012 Wiley Periodicals, Inc.
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fogarty International Center Program for Training and Research in Population and Health
Grant number Fogarty International Center Program for Training and Research in Population and Health: D43TW/HD00627
Fogarty International Center Program for Training and Research in Population and Health: UNC/UNIFESP 5-53284
Date 2012-12-01
Published in Molecular Reproduction and Development. Hoboken: Wiley-Blackwell, v. 79, n. 12, p. 832-842, 2012.
ISSN 1040-452X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 832-842
Origin http://dx.doi.org/10.1002/mrd.22119
Access rights Closed access
Type Article
Web of Science ID WOS:000312308500004
URI http://repositorio.unifesp.br/handle/11600/35540

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