Effect of Adenosine on Injury Caused by lschemia and Reperfusion in Rats: Functional and Morphologic Study

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Haddad, Michel [UNIFESP]
Miranda-Ferreira, Regiane [UNIFESP]
Taha, Nabiha Saadi Abrahão [UNIFESP]
Maldonado, Valente Calatayud [UNIFESP]
Daroz, Rafael René [UNIFESP]
Daud, Meliane de Oliveira [UNIFESP]
Silva Neto, Jose Lima [UNIFESP]
Muniz, Daniella Araujo [UNIFESP]
Silva, Paulo César da [UNIFESP]
Monteiro, Hugo Pequeno [UNIFESP]
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To study whether treatment with adenosine (ADO), an agonist of adenosine receptors, attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), we treated rats with ADO (15 mg/kg or saline solution (SS) intravenously before 60 minutes occlusion of the superior mesenteric artery (I) and/or 120 minutes after its release (R). After I or I/R, isolated jejunal segments (2 cm) were mounted in an organ bath to study nerve-mediated contractions stimulated by electrical pulses or KCI with the use of a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Compared with the sham group, jejunal contractions were reduced in I+SS and IR+SS but similar after treatment with ADO (I+ADO and IR+ADO groups). We concluded that rat jejunal enteric nerves were damaged in I+SS and IR+SS but not in the I+ADO and IR+ADO groups. These results suggested that ADO attenuated intestinal dysfunction due to I and R.
Transplantation Proceedings. New York: Elsevier B.V., v. 44, n. 8, p. 2317-2320, 2012.