Inflammatory milieu as an early marker of kidney injury in offspring rats from diabetic mothers

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Correa-Costa, Matheus
Landgraf, Maristella A.
Cavanal, Maria de Fátima [UNIFESP]
Semedo, Patricia [UNIFESP]
Vieira, Daniel Augusto Ghiraldini [UNIFESP]
Marco, Davi Tjhio Kolar de [UNIFESP]
Hirata, Aparecida Emiko [UNIFESP]
Câmara, Niels Olsen Saraiva [UNIFESP]
Gil, Frida Zaladek [UNIFESP]
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The present study investigated the early presence of inflammatory response in renal tissue of young offspring from diabetic mothers. the effect of L-arginine (L-arg) supplementation was also investigated. the offspring was divided into four groups: group CO (controls); group DO (diabetic offspring); group CA (CO receiving 2% L-arg solution) and group DA (DO receiving the 2% L-arg solution). Glycemia, arterial pressure and renal function were evaluated; gene and protein expression of pro-inflammatory cytokines were also measured. Blood pressure levels were significantly increased in 2 and 6 month-old DO rats, whereas L-arg administration caused a significant decrease in the DA group, at both ages. DO rats showed a significantly blunted glycemic response to exogenous insulin. in 2 month-old DO animals, renal protein expression of pro-inflammatory molecules was significantly increased. At six months of age, we also observed an increase in gene expression of pro-inflammatory molecules, whereas L-arg supplementation prevented this increase at both ages. Our data suggest that activation of inflammatory pathways is present early in the kidney of DO rats, and that L-arg can attenuate the expression of these markers of tissue inflammation. Our results also reinforce the concept that intrauterine environmental factors are a fundamental determinant in the development of metabolic and vascular diseases later in life. (C) 2012 Elsevier B.V. All rights reserved.
European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 689, n. 1-3, p. 233-240, 2012.