Lipid fingerprinting in women with early-onset preeclampsia: A first look

Abstract

Objectives: the aim of this preliminary study was to characterize the plasma lipid profiling of women with preeclampsia.Design and methods: Plasma samples of 8 pregnant women with early-onset preeclampsia and 8 normal pregnant women were evaluated. Lipids were extracted from plasma using the Bligh-Dyer protocol. the extracts were subjected to MALDI-MS. Data matrix was exported for partial least squares discriminant analysis (PLS-DA) and a parameter VIP was employed to reflect the variable importance in the discriminant analysis. the major discriminant variables were selected and underwent to Mann-Whitney U test.Results: A total of 1290 ions were initially identified and twelve m/z signals were highlighted as the most important lipids for the discrimination of patients with preeclampsia. the identification of these differential lipids was carried out through Lipid Database Search.Conclusions: the main classes identified were glycerophosphocholines [GP01], glycerophosphoserines [GP03], glycerophosphoglycerols [GP04], glycosyldiradylglycerols [GL05] and glycerophosphates [GP10]. (C) 2012 the Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.