Lipid fingerprinting in women with early-onset preeclampsia: A first look

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2012-07-01
Autores
De Oliveira, Leandro [UNIFESP]
Camara, Niels Olsen S.
Bonetti, Tatiana [UNIFESP]
Lo Turco, Edson G. [UNIFESP]
Bertolla, Ricardo Pimenta [UNIFESP]
Moron, Antonio F. [UNIFESP]
Sass, Nelson [UNIFESP]
Cotrim Guerreiro Da Silva, Ismael Dale [UNIFESP]
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Objectives: the aim of this preliminary study was to characterize the plasma lipid profiling of women with preeclampsia.Design and methods: Plasma samples of 8 pregnant women with early-onset preeclampsia and 8 normal pregnant women were evaluated. Lipids were extracted from plasma using the Bligh-Dyer protocol. the extracts were subjected to MALDI-MS. Data matrix was exported for partial least squares discriminant analysis (PLS-DA) and a parameter VIP was employed to reflect the variable importance in the discriminant analysis. the major discriminant variables were selected and underwent to Mann-Whitney U test.Results: A total of 1290 ions were initially identified and twelve m/z signals were highlighted as the most important lipids for the discrimination of patients with preeclampsia. the identification of these differential lipids was carried out through Lipid Database Search.Conclusions: the main classes identified were glycerophosphocholines [GP01], glycerophosphoserines [GP03], glycerophosphoglycerols [GP04], glycosyldiradylglycerols [GL05] and glycerophosphates [GP10]. (C) 2012 the Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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Clinical Biochemistry. Oxford: Pergamon-Elsevier B.V., v. 45, n. 10-11, p. 852-855, 2012.
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