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dc.contributor.authorLima, Cilene Reboucas de [UNIFESP]
dc.contributor.authorSantos Júnior, José de Arimatea [UNIFESP]
dc.contributor.authorPinto Nazário, Afonso Celso [UNIFESP]
dc.contributor.authorMichelacci, Yara Maria [UNIFESP]
dc.date.accessioned2016-01-24T14:27:23Z
dc.date.available2016-01-24T14:27:23Z
dc.date.issued2012-07-01
dc.identifierhttp://dx.doi.org/10.1016/j.bbagen.2012.04.010
dc.identifier.citationBiochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1820, n. 7, p. 1009-1019, 2012.
dc.identifier.issn0304-4165
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35010
dc.description.abstractBackground: Fibroadenoma is the most common breast tumor in young women, and its growth and metabolism may be under hormonal control. in the present paper we described the proteoglycan (PG) composition and synthesis rate of normal breast and fibroadenoma during the menstrual cycle.Methods: Samples of fibroadenoma and adjacent normal breast tissue were obtained at surgery. PGs were characterized by agarose gel electrophoresis and enzymatic degradation with glycosaminoglycan (GAG) lyases, and immunolocalized by confocal microscopy. To assess the synthesis rate, PGs were metabolic labeled by S-35-sulfate.Results: the concentration of PGs in normal breast was higher during the secretory phase. Fibroadenoma contained and synthesized more PGs than their paired controls, but the PG concentrations varied less with the menstrual cycle and, in contrast to normal tissue, peaked in the proliferative phase. the main mammary GAGs are heparan sulfate (HS, 71%-74%) and dermatan sulfate (DS, 26%-29%). the concentrations of both increased in fibroadenoma, but DS increased more, becoming 35%-37% of total. the DS chains contained more beta-D-glucuronic acid (IdoUA/GlcUA ratios were >10 in normal breast and 2-7 in fibroadenoma). the S-35-sulfate incorporation rate revealed that the in vitro synthesis rate of DS was higher than HS. Decorin was present in both tissues, while versican was found only in fibroadenoma.Conclusions: in normal breast, the PG concentration varied with the menstrual cycle. It was increased in fibroadenoma, especially DS.General significancePGs are increased in fibroadenoma, but their concentrations may be less sensitive to hormonal control. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipSociedade Paulista para o Desenvolvimento da Medicina (SPDM), São Paulo, SP, Brazil
dc.format.extent1009-1019
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochimica Et Biophysica Acta-general Subjects
dc.rightsAcesso aberto
dc.subjectFibroadenomaen
dc.subjectProteoglycanen
dc.subjectMenstrual cycleen
dc.subjectNormal breasten
dc.subjectDecorinen
dc.subjectVersicanen
dc.titleChanges in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycleen
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Bioquim, Escola Paulista Med, Disciplina Biol Mol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Ginecol, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Bioquim, Escola Paulista Med, Disciplina Biol Mol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Ginecol, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.identifier.fileWOS000305366100026.pdf
dc.identifier.doi10.1016/j.bbagen.2012.04.010
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000305366100026


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