Effectiveness, against tuberculosis, of pseudo-ternary complexes: Peptide-DNA-cationic liposome

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Date
2012-05-01Author
Rosada, Rogério Silva
Silva, Cello Lopes
Santana, Maria Helena Andrade
Nakaie, Clovis Ryuichi [UNIFESP]
De la Torre, Lucimara Gaziola
Type
ArtigoISSN
0021-9797Is part of
Journal of Colloid and Interface ScienceDOI
10.1016/j.jcis.2011.09.040Metadata
Show full item recordAbstract
We report the effects of a synthetic peptide designed to act as a nuclear localization signal on the treatment of tuberculosis. the peptide contains 21 amino acid residues with the following specific domains: nuclear localization signal from SV 40T, cationic shuttle sequence, and cysteamide group at the C-terminus. the peptide was complexed with the plasmid DNAhsp65 and incorporated into cationic liposomes, forming a pseudo-ternary complex. the same cationic liposomes, composed of egg chicken L-alpha-phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane, and 1,2-dioleoyl-3-trimethylammonium-propane (2:1:1 M), were previously evaluated as a gene carrier for tuberculosis immunization protocols with DNAhsp65. the pseudo-ternary complex presented a controlled size (250 nm), spherical-like shape, and various lamellae in liposomes as evaluated by transmission electron microscopy. An assay of fluorescence probe accessibility confirmed insertion of the peptide/DNA into the liposome structure. Peptide addition conferred no cytotoxicity in vitro, and similar therapeutic effects against tuberculosis were seen with four times less DNA compared with naked DNA treatment. Taken together, the results indicate that the pseudo-ternary complex is a promising gene vaccine for tuberculosis treatment. This work contributes to the development of multifunctional nanostructures in the search for strategies for in vivo DNA delivery. (C) 2011 Elsevier Inc. All rights reserved.
Citation
Journal of Colloid and Interface Science. San Diego: Academic Press Inc Elsevier Science, v. 373, p. 102-109, 2012.Keywords
Cationic liposomeDNA delivery
Nuclear localization signal
Tuberculosis
Gene vaccine
Infectious disease
Sponsorship
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Collections
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