B-1 lymphocytes differentiate into functional osteoclast-like cells

B-1 lymphocytes differentiate into functional osteoclast-like cells

Author Pugliese, Livia Souza Autor UNIFESP Google Scholar
Gonçalves-Zillo, Thais Oliveira Autor UNIFESP Google Scholar
Popi, Ana Flavia Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Pesquero, Joao Bosco Autor UNIFESP Google Scholar
Lopes, Jose Daniel Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The existence of murine peritoneal osteoclast precursors has been already described. Also, recent reports evidenced an interplay between B lymphocytes and osteoclasts development. B-1 cells comprise a B-lymphocyte subset that resides mostly in pleural and peritoneal cavities. It has been demonstrated that B-1 cells can differentiate into mononuclear phagocytes and form multinucleated giant cells. Based on these findings, we investigated the role of B-1 lymphocytes in bone resorption and osteoclastogenesis. in vivo experimental periodontitis induced in B-1 deficient Xid mice demonstrated that bone resorption is impaired in these animals. However, reconstitution of Xid mice with B-1 cells increased bone resorption to near Balb/c values. B-1 cell derived phagocytes express the receptor activator of nuclear factor-kappa B (RANK) and the macrophage colony-stimulating factor receptor (M-CSFR). When cultured with RANK-ligand (RANKL) and M-CSF, B-1 cells became tartrate resistant acid phosphatase (TRAP) positive multinucleated cells, a typical osteoclast phenotype. Lacunae formation was observed when cells were cultivated onto a calcium phosphate analog, indicating functional differentiation of B1 cells into osteoclast-like cells. the dynamics of their IgM expression showed that this lymphoid marker was downregulated along the differentiation of B-1 lymphocytes into osteoclasts. Our results unveiled the first evidence that B-1 cells have a role in osteoclastogenesis and bone resorption and offer new insights in the relationship between bone and lymphoid cells. (C) 2011 Elsevier GmbH. All rights reserved.
Keywords B-1 lymphocytes
Bone resorption
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 06/52985-7
FAPESP: 07/51501-9
Date 2012-03-01
Published in Immunobiology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 217, n. 3, p. 336-344, 2012.
ISSN 0171-2985 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 336-344
Origin http://dx.doi.org/10.1016/j.imbio.2011.07.014
Access rights Closed access
Type Article
Web of Science ID WOS:000302109700006
URI http://repositorio.unifesp.br/handle/11600/34677

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