Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage

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Date
2012-03-01Author
Guimaraes, Natalia S. S.
Mello, Joyce C.
Paiva, Juliana S.
Bueno, Paula C. P.
Berretta, Andresa A.
Torquato, Ricardo José Soares [UNIFESP]
Nantes, Iseli L.
Rodrigues, Tiago
Type
ArtigoISSN
0278-6915Is part of
Food and Chemical ToxicologyDOI
10.1016/j.fct.2011.11.014Metadata
Show full item recordAbstract
Baccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. in this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). the GEBd was prepared by fractionated percolation using propylene glycol as solvent. the total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe2+ chelating activity. GEBd decreased the basal H2O2 generation and the Fe2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis. (C) 2011 Elsevier B.V. All rights reserved.
Citation
Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 50, n. 3-4, p. 1091-1097, 2012.Keywords
Baccharis dracunculifoliaAntioxidant
Free radicals
Hepatoprotection
Mitochondria
Oxidative stress
Sponsorship
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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