Bcl-2 Modulates Endoplasmic Reticulum and Mitochondrial Calcium Stores in PC12 Cells

Show simple item record

dc.contributor.author Hirata, Hanako [UNIFESP]
dc.contributor.author Lopes, Guiomar S. [UNIFESP]
dc.contributor.author Jurkiewicz, Aron [UNIFESP]
dc.contributor.author Garcez-do-Carmo, Lucia [UNIFESP]
dc.contributor.author Smaili, Soraya Soubhi [UNIFESP]
dc.date.accessioned 2016-01-24T14:17:51Z
dc.date.available 2016-01-24T14:17:51Z
dc.date.issued 2012-02-01
dc.identifier http://dx.doi.org/10.1007/s11064-011-0600-5
dc.identifier.citation Neurochemical Research. New York: Springer/plenum Publishers, v. 37, n. 2, p. 238-243, 2012.
dc.identifier.issn 0364-3190
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/34571
dc.description.abstract Endoplasmic reticulum (ER) and mitochondria are intracellular organelles and their interactions are directly involved in different processes such as Ca2+ signaling in cell survival and death mechanisms. Bcl-2 is an anti-apoptotic protein intrinsically related to ER and mitochondria, modulating Ca2+ content in these organelles. We investigated the effects of Bcl-2 overexpression on ER and mitochondrial Ca2+ dynamics in PC12 cells. Bcl-2 overexpressing and control cells were loaded with Fura 2/AM and stimulated with different drugs. Results showed that in Bcl-2 cells, ACh induced a lower Ca2+ response compared to control. Ca2+ release induced by TG was decreased in Bcl-2 cells, however, it was greater in Caff induced Ca2+ rise. in addition, FCCP induced a higher Ca2+ release in Bcl-2 cells. These results suggest that Bcl-2 overexpression modulate the ER Ca2+ pools differently and the release of ER Ca2+ may increase mitochondrial Ca2+ accumulation. These alterations of intracellular Ca2+ stores are important mechanisms for the control of Ca2+ signaling. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 238-243
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof Neurochemical Research
dc.rights Acesso restrito
dc.subject PC12 en
dc.subject Bcl-2 en
dc.subject Calcium en
dc.subject ER en
dc.subject Mitochondria en
dc.subject ACh en
dc.title Bcl-2 Modulates Endoplasmic Reticulum and Mitochondrial Calcium Stores in PC12 Cells en
dc.type Artigo
dc.rights.license http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo UNIFESP EPM, Dept Farmacol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP EPM, Dept Farmacol, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1007/s11064-011-0600-5
dc.description.source Web of Science
dc.identifier.wos WOS:000302404100002



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account