Cytogenetic instability of dental pulp stem cell lines

Date
2012-02-01Author
Duailibi, Monica Talarico [UNIFESP]
Kulikowski, Leslie Domenici
Duailibi, Silvio Eduardo [UNIFESP]
Nunes Lipay, Monica Vannucci [UNIFESP]
Melaragno, Maria Isabel
Ferreira, Lydia Masako [UNIFESP]
Vacanti, Joseph Phillip
Yelick, Pamela Crotty
Type
ArtigoISSN
1567-2379Is part of
Journal of Molecular HistologyDOI
10.1007/s10735-011-9373-zMetadata
Show full item recordAbstract
Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. the heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.
Citation
Journal of Molecular Histology. Dordrecht: Springer, v. 43, n. 1, p. 89-94, 2012.Keywords
Chromosomal abnormalitiesCell transplantation
Tooth tissue engineering
Human dental stem cells
Sponsorship
INCT-Biofabrication InstituteConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Rede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED
NIH/NIDCR
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