Characteristics of Muscle Fibers in Rats with Limb Movements during Sleep after Spinal Cord Injury

Characteristics of Muscle Fibers in Rats with Limb Movements during Sleep after Spinal Cord Injury

Author Esteves, Andrea M. Autor UNIFESP Google Scholar
Squarcini, Camila F. R. Autor UNIFESP Google Scholar
Lancellotti, Carmen L. P. Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Mello, Marco T. de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fac Ciencias Med Santa Casa São Paulo
Abstract Background/Aims: Previous studies have demonstrated that spinal cord injury (SCI) results in changes in sleep patterns through increased arousals and limb movements during sleep. Dramatic changes in muscle myosin heavy-chain isoforms have also been reported. the aim of this study was to investigate the characteristics of muscle fibers after SCI in rats with limb movements during sleep. Methods: Forty male Wistar rats were divided into four groups: SHAM, SCI 3, 7 and 15 days. Animals were subjected to electrode insertion surgery, 24-hour baseline sleep recording, SCI, and subsequent sleep recording for 3, 7, or 15 consecutive days. in addition, the gastrocnemius muscle and spinal cord were collected for histopathological/histochemical analyses. Results: Our results indicate a rapid and progressive decrease in the cross-sectional area of type I fibers in the gastrocnemius muscle (35.76-24.74 mu m(2)) after SCI. Additionally, we found SCI-induced changes in sleep patterns. Following SCI, we also observed limb movements in sleeping rats, as well as significant negative moderate correlations between type I fibers and limb movement. Conclusion: Our study strengthened the hypothesis by correlation between changes in types of muscle fiber (decline in type I fibers) and an increase in limb movements during sleep after SCI. Copyright (c) 2012 S. Karger AG, Basel
Keywords Movement disorders
Sleep
Sleep disorders
Spinal cord
Muscle biopsy
Language English
Sponsor Associacao Fundo Incentivo a Pesquisa (AFIP)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Centro de Estudo Multidiciplinar em Sonolencia e Acidentes (CEMSA)
Centro de Estudos em Psicobiologia e Exercicio (CEPE)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 03/06297-3
FAPESP: CEPID 98/143033
Date 2012-01-01
Published in European Neurology. Basel: Karger, v. 67, n. 2, p. 107-115, 2012.
ISSN 0014-3022 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 107-115
Origin http://dx.doi.org/10.1159/000334102
Access rights Closed access
Type Article
Web of Science ID WOS:000300305500008
URI http://repositorio.unifesp.br/handle/11600/34496

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