Implications for the use of acid preservatives in 24-hour urine for measurements of high demand biochemical analytes in clinical laboratories
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2011-11-20
Autores
Feres, Márcia Cristina [UNIFESP]
Bini, Rolando [UNIFESP]
De Martino, Maria Cristina
Biagini, Simone P.
Sousa, Altay Lino de [UNIFESP]
Campana, Paulo G.
Tufik, Sergio [UNIFESP]
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Background: Evaluate the level of interference of biochemists dosages in the 24-hour urine using or not the 6 mol/l HCl acid in different concentrations and conditions and its implications in the most demanded analytes in clinical laboratory.Methods: Twenty-two volunteers collected three 24-hour urine in 3 conditions: with 5 ml/l and 20 ml/l of 6 mol/l HCl in the container, and without acid preservative. the samples collected without preservative were separated in aliquots and added 5 ml/l of 6 mol/l HCl after 24 h. Analytes, uric acid creatinine, urea, chlorides, glucose, magnesium, sodium, potassium, microalbumin, proteins, amylase, aldosterone, calcium, cortisol, phosphorus, citric acid, oxalate, and metanephrines, were determined.Results: Uric acid, glucose, microalbumin, protein, amylase and aldosterone showed that %CV ranging from 16 to 57% in the presence of acid preservative. Analytes that need acid preservative cortisol, citric acid and oxalate showed %CV ranging from 6 to 27% with r=0.66, r=0.77, r=0.70 respectively provided 5 ml/l after delivery and r=0.31, r=0.70 and r=0.48 without preservative acid when compared with the gold standard (with 20 ml/l of 6 mol/l HCl).Conclusions: Glucose, microalbumin, protein, amylase and aldosterone urinary did not show good performance in the presence of acid preservative. Analytes that need acid preservative showed variation acceptable in condition 5 ml/l of 6 mol/l HCl added after 24 h. (C) 2011 Elsevier B.V. All rights reserved.
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Clinica Chimica Acta. Amsterdam: Elsevier B.V., v. 412, n. 23-24, p. 2322-2325, 2011.