Implications for the use of acid preservatives in 24-hour urine for measurements of high demand biochemical analytes in clinical laboratories

Implications for the use of acid preservatives in 24-hour urine for measurements of high demand biochemical analytes in clinical laboratories

Author Feres, Márcia Cristina Autor UNIFESP Google Scholar
Bini, Rolando Autor UNIFESP Google Scholar
De Martino, Maria Cristina Google Scholar
Biagini, Simone P. Google Scholar
Sousa, Altay Lino de Autor UNIFESP Google Scholar
Campana, Paulo G. Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Assoc Fundo Incent Psicofarmacol
Abstract Background: Evaluate the level of interference of biochemists dosages in the 24-hour urine using or not the 6 mol/l HCl acid in different concentrations and conditions and its implications in the most demanded analytes in clinical laboratory.Methods: Twenty-two volunteers collected three 24-hour urine in 3 conditions: with 5 ml/l and 20 ml/l of 6 mol/l HCl in the container, and without acid preservative. the samples collected without preservative were separated in aliquots and added 5 ml/l of 6 mol/l HCl after 24 h. Analytes, uric acid creatinine, urea, chlorides, glucose, magnesium, sodium, potassium, microalbumin, proteins, amylase, aldosterone, calcium, cortisol, phosphorus, citric acid, oxalate, and metanephrines, were determined.Results: Uric acid, glucose, microalbumin, protein, amylase and aldosterone showed that %CV ranging from 16 to 57% in the presence of acid preservative. Analytes that need acid preservative cortisol, citric acid and oxalate showed %CV ranging from 6 to 27% with r=0.66, r=0.77, r=0.70 respectively provided 5 ml/l after delivery and r=0.31, r=0.70 and r=0.48 without preservative acid when compared with the gold standard (with 20 ml/l of 6 mol/l HCl).Conclusions: Glucose, microalbumin, protein, amylase and aldosterone urinary did not show good performance in the presence of acid preservative. Analytes that need acid preservative showed variation acceptable in condition 5 ml/l of 6 mol/l HCl added after 24 h. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Analytes
Preservative
Acidification
24-h urine
6 mol/l HCl
Collection
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Associacao Fundo de Pesquisa a Psicobiologia (AFIP)
Date 2011-11-20
Published in Clinica Chimica Acta. Amsterdam: Elsevier B.V., v. 412, n. 23-24, p. 2322-2325, 2011.
ISSN 0009-8981 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 2322-2325
Origin http://dx.doi.org/10.1016/j.cca.2011.08.033
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000296686100052
URI http://repositorio.unifesp.br/handle/11600/34229

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