Cytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)

Cytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)

Author Silva, Wemeson F. da Autor UNIFESP Google Scholar
Egami, Mizue Imoto Autor UNIFESP Google Scholar
Santos, Antenor A. Google Scholar
Antoniazzi, Marta M. Google Scholar
Silva, Marcelo Autor UNIFESP Google Scholar
Gutierre, Robson C. Autor UNIFESP Google Scholar
Paiva, Maria Jose R. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNASP
Butantan Inst
Fishery Inst
Abstract The cytochemical, immunocytochemical and ultrastructural characteristics of leukocytes and thrombocytes in the peripheral blood of the fat snook (Centropomus paralellus) - a fish occurring in Brazil were investigated. the cytochemical methods were performed to demonstrate four enzymatic reactions o-toluidine-hydrogen peroxide, naphtol AS-MX phosphate, naphtol AS-BI phosphate and alpha-naphtil acetate to detect myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP) and non-specific esterase (alpha-NAE), respectively and two non-enzymatic ones Periodic-Acid Schiff (PAS) and Sudan black B (SBB) to detect the occurrence of glycogen and phospholipids, respectively. Immunocytochemical method utilizing polyclonal rabbit antibody against mammal metalloproteinases (MMPs) 2 and 9 were done. Standard method for Electron Microscopy (EM) was applied for the ultrastructural study. the cytochemical reactions were positive in neutrophils for MPO, ACP, alpha-NAE, glycogen and phospholipids; in lymphocytes for ACP and alpha-NAE; in monocytes for ACP and alpha-NAE and in thrombocytes for ACP, alpha-NAE and glycogen. Only neutrophils were positive for MMPs 2 and 9, and none of the cells studied were positive for ALP. Ultrastructurally: 1) neutrophil showed a spherical shape with a spherical, indented or lobulated euchromatic nucleus, and cytoplasm containing granules of varied sizes and mitochondria of varied shapes and sizes. the nucleus/cytoplasm relation and the size of granules suggest neutrophil maturation in peripheral blood; 2) lymphocytes showed partially heterochromatic nucleus and minimal cytoplasm; 3) monocytes had long cytoplasmic projections, an indented nucleus, evident nucleolus and cytoplasm with granules of varied sizes and vacuoles; 4) thrombocytes were predominantly elliptical or roughly spherical in shape, had a partially heterochromatic nucleus and cytoplasm containing electron-dense granules, intricate canalicular system and vacuoles occasionally holding phagocytic material. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Cytochemistry
Immunocytochemistry
Ultrastructure
Leukocytes
Centropomus parallelus
Language English
Date 2011-10-01
Published in Fish & Shellfish Immunology. London: Academic Press Ltd- Elsevier B.V., v. 31, n. 4, p. 571-577, 2011.
ISSN 1050-4648 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 571-577
Origin http://dx.doi.org/10.1016/j.fsi.2011.07.019
Access rights Closed access
Type Article
Web of Science ID WOS:000295020000008
URI http://repositorio.unifesp.br/handle/11600/34106

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