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dc.contributor.authorSouza, Denise C. F. [UNIFESP]
dc.contributor.authorSucupira, Maria Cecilia A. [UNIFESP]
dc.contributor.authorBrindeiro, Rodrigo M.
dc.contributor.authorFernandez, Jose Carlos C.
dc.contributor.authorSabino, Ester C.
dc.contributor.authorInocencio, Lilian A. [UNIFESP]
dc.contributor.authorDiaz, Ricardo Sobhie [UNIFESP]
dc.date.accessioned2016-01-24T14:17:13Z
dc.date.available2016-01-24T14:17:13Z
dc.date.issued2011-09-21
dc.identifierhttp://dx.doi.org/10.1186/1758-2652-14-45
dc.identifier.citationJournal of the International Aids Society. London: Biomed Central Ltd, v. 14, 6 p., 2011.
dc.identifier.issn1758-2652
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34054
dc.description.abstractThe Brazilian network for genotyping is composed of 21 laboratories that perform and analyze genotyping tests for all HIV-infected patients within the public system, performing approximately 25,000 tests per year. We assessed the interlaboratory and intralaboratory reproducibility of genotyping systems by creating and implementing a local external quality control evaluation. Plasma samples from HIV-1-infected individuals (with low and intermediate viral loads) or RNA viral constructs with specific mutations were used. This evaluation included analyses of sensitivity and specificity of the tests based on qualitative and quantitative criteria, which scored laboratory performance on a 100-point system. Five evaluations were performed from 2003 to 2008, with 64% of laboratories scoring over 80 points in 2003, 81% doing so in 2005, 56% in 2006, 91% in 2007, and 90% in 2008 (Kruskal-Wallis, p = 0.003). Increased performance was aided by retraining laboratories that had specific deficiencies. the results emphasize the importance of investing in laboratory training and interpretation of DNA sequencing results, especially in developing countries where public (or scarce) resources are used to manage the AIDS epidemic.en
dc.format.extent6
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofJournal of the International Aids Society
dc.rightsAcesso aberto
dc.titleThe Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programmeen
dc.typeArtigo
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionMinist Hlth
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionInst Oswaldo Cruz
dc.contributor.institutionFundacao Prosangue
dc.description.affiliationUniversidade Federal de São Paulo, Lab Retrovirol, São Paulo, Brazil
dc.description.affiliationMinist Hlth, Brazilian STD AIDS & Viruses Hepatitis Dept, Brasilia, DF, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Mol Virol Lab, Rio de Janeiro, Brazil
dc.description.affiliationInst Oswaldo Cruz, Lab AIDS & Mol Immunol, BR-20001 Rio de Janeiro, Brazil
dc.description.affiliationFundacao Prosangue, Hemoctr São Paulo, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Lab Retrovirol, São Paulo, Brazil
dc.identifier.fileWOS000296354200001.pdf
dc.identifier.doi10.1186/1758-2652-14-45
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000296354200001


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