The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme

The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme

Author Souza, Denise C. F. Autor UNIFESP Google Scholar
Sucupira, Maria Cecilia A. Autor UNIFESP Google Scholar
Brindeiro, Rodrigo M. Google Scholar
Fernandez, Jose Carlos C. Google Scholar
Sabino, Ester C. Google Scholar
Inocencio, Lilian A. Autor UNIFESP Google Scholar
Diaz, Ricardo Sobhie Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Minist Hlth
Universidade Federal do Rio de Janeiro (UFRJ)
Inst Oswaldo Cruz
Fundacao Prosangue
Abstract The Brazilian network for genotyping is composed of 21 laboratories that perform and analyze genotyping tests for all HIV-infected patients within the public system, performing approximately 25,000 tests per year. We assessed the interlaboratory and intralaboratory reproducibility of genotyping systems by creating and implementing a local external quality control evaluation. Plasma samples from HIV-1-infected individuals (with low and intermediate viral loads) or RNA viral constructs with specific mutations were used. This evaluation included analyses of sensitivity and specificity of the tests based on qualitative and quantitative criteria, which scored laboratory performance on a 100-point system. Five evaluations were performed from 2003 to 2008, with 64% of laboratories scoring over 80 points in 2003, 81% doing so in 2005, 56% in 2006, 91% in 2007, and 90% in 2008 (Kruskal-Wallis, p = 0.003). Increased performance was aided by retraining laboratories that had specific deficiencies. the results emphasize the importance of investing in laboratory training and interpretation of DNA sequencing results, especially in developing countries where public (or scarce) resources are used to manage the AIDS epidemic.
Language English
Date 2011-09-21
Published in Journal of the International Aids Society. London: Biomed Central Ltd, v. 14, 6 p., 2011.
ISSN 1758-2652 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 6
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000296354200001

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