Application of Real-Time PCR Stool Assay for Helicobacter pylori Detection and Clarithromycin Susceptibility Testing in Brazilian Children

Nenhuma Miniatura disponível
Data
2011-08-01
Autores
Scaletsky, Isabel C. A. [UNIFESP]
Aranda, Katia R. S. [UNIFESP]
Garcia, Gabriela T. [UNIFESP]
Goncalves, Manoel E. P.
Cardoso, Silvia R.
Iriya, Kiyoshi
Silva, Neusa P. [UNIFESP]
Orientadores
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Background: Helicobacter pylori ClariRes assay is a novel commercially available real-time PCR assay allowing H. pylori detection and clarithromycin susceptibility testing in either gastric biopsy or stool specimens.Objective: the aim of this study was to validate the novel biprobe real-time assay in stool specimens from 217 dyspeptic children.Methods: DNA from gastric biopsies and stool specimens were obtained and submitted to the biprobe real time assay for H. pylori detection and clarithromycin susceptibility testing.Results: the sensitivity, specificity, and test accuracy were 69, 100 and 93.9% for the detection of H. pylori infection and 83.3, 100 and 95.6%, for detection of clarithromycin resistance.Conclusion: This assay proved to be appropriate for H. pylori clarithromycin susceptibility testing, particularly in children populations where a high prevalence of clarithromycin-resistant strains is suspected.
Descrição
Citação
Helicobacter. Malden: Wiley-Blackwell, v. 16, n. 4, p. 311-315, 2011.
Coleções